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Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62
Protein import into the nucleus is a multistep process that requires the activities of several cytosolic factors. In this study we have purified a cytosolic factor that interacts with the nuclear pore complex glycoprotein p62. Isolation involved biochemical complementation of cytosol depleted of thi...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1995
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120498/ https://www.ncbi.nlm.nih.gov/pubmed/7744965 |
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collection | PubMed |
description | Protein import into the nucleus is a multistep process that requires the activities of several cytosolic factors. In this study we have purified a cytosolic factor that interacts with the nuclear pore complex glycoprotein p62. Isolation involved biochemical complementation of cytosol depleted of this activity by preadsorption with recombinant p62 and the use of a novel flow cytometry-based assay for quantitation of nuclear import. The purified activity (NTF2) is an apparent dimer of approximately 14-kD subunits and is present at approximately 10(6) copies per cell. We obtained a cDNA encoding NTF2 and showed that the recombinant protein restores transport activity to p62-pretreated cytosol. Our data suggest that NTF2 acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. NTF2 interacts with at least one additional cytosolic transport activity, indicating that it could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. |
format | Text |
id | pubmed-2120498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21204982008-05-01 Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 J Cell Biol Articles Protein import into the nucleus is a multistep process that requires the activities of several cytosolic factors. In this study we have purified a cytosolic factor that interacts with the nuclear pore complex glycoprotein p62. Isolation involved biochemical complementation of cytosol depleted of this activity by preadsorption with recombinant p62 and the use of a novel flow cytometry-based assay for quantitation of nuclear import. The purified activity (NTF2) is an apparent dimer of approximately 14-kD subunits and is present at approximately 10(6) copies per cell. We obtained a cDNA encoding NTF2 and showed that the recombinant protein restores transport activity to p62-pretreated cytosol. Our data suggest that NTF2 acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. NTF2 interacts with at least one additional cytosolic transport activity, indicating that it could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. The Rockefeller University Press 1995-05-02 /pmc/articles/PMC2120498/ /pubmed/7744965 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 |
title | Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 |
title_full | Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 |
title_fullStr | Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 |
title_full_unstemmed | Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 |
title_short | Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 |
title_sort | identification of ntf2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120498/ https://www.ncbi.nlm.nih.gov/pubmed/7744965 |