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A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells

The CHO cell mutant FD 1.3.25 exhibits both increased accumulation and altered distribution of endocytosed fluid phase tracers. Neither the rate of tracer internalization nor the kinetics of recycling from early endosomes was affected, but exocytosis from late endocytic compartments appeared to be d...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120546/
https://www.ncbi.nlm.nih.gov/pubmed/7657694
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description The CHO cell mutant FD 1.3.25 exhibits both increased accumulation and altered distribution of endocytosed fluid phase tracers. Neither the rate of tracer internalization nor the kinetics of recycling from early endosomes was affected, but exocytosis from late endocytic compartments appeared to be decreased in the mutant. Endocytosed tracer moved more rapidly to the cell poles in FD1.3.25 than in wild type cells. An abundant 36-kD polypeptide was found associated with taxol-polymerized microtubules in preparations from wild type and mutant; in the former but not the latter this polypeptide could be dissociated by incubation of the microtubules in ATP or high salt. The 36-kD polypeptide co- electrophoresed in two dimensions with the monomer of the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Analysis of cDNA clones showed that the mutant is heterozygous for this enzyme, with approximately 25% of the GAPDH RNA containing a single nucleotide change resulting in substitution of Ser for Pro234, a residue that is conserved throughout evolution. Stable transfectants of wild type cells expressing the mutant monomer at approximately 15% of the total enzyme exhibited the various changes in endocytosis observed in FD1.3.25.
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spelling pubmed-21205462008-05-01 A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells J Cell Biol Articles The CHO cell mutant FD 1.3.25 exhibits both increased accumulation and altered distribution of endocytosed fluid phase tracers. Neither the rate of tracer internalization nor the kinetics of recycling from early endosomes was affected, but exocytosis from late endocytic compartments appeared to be decreased in the mutant. Endocytosed tracer moved more rapidly to the cell poles in FD1.3.25 than in wild type cells. An abundant 36-kD polypeptide was found associated with taxol-polymerized microtubules in preparations from wild type and mutant; in the former but not the latter this polypeptide could be dissociated by incubation of the microtubules in ATP or high salt. The 36-kD polypeptide co- electrophoresed in two dimensions with the monomer of the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Analysis of cDNA clones showed that the mutant is heterozygous for this enzyme, with approximately 25% of the GAPDH RNA containing a single nucleotide change resulting in substitution of Ser for Pro234, a residue that is conserved throughout evolution. Stable transfectants of wild type cells expressing the mutant monomer at approximately 15% of the total enzyme exhibited the various changes in endocytosis observed in FD1.3.25. The Rockefeller University Press 1995-09-01 /pmc/articles/PMC2120546/ /pubmed/7657694 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells
title A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells
title_full A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells
title_fullStr A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells
title_full_unstemmed A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells
title_short A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells
title_sort mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in cho cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120546/
https://www.ncbi.nlm.nih.gov/pubmed/7657694