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COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus

The noncatalytic domain of the human T cell protein tyrosine phosphatase (TCPTP) is alternatively spliced to generate a 45-kD form, p45TC, and a 48-kD form, p48TC (Champion-Arnaud et al., 1991; Mosinger et al., 1992). This manuscript concerns structural motifs in the noncatalytic segment of the enzy...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120615/
https://www.ncbi.nlm.nih.gov/pubmed/7593185
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description The noncatalytic domain of the human T cell protein tyrosine phosphatase (TCPTP) is alternatively spliced to generate a 45-kD form, p45TC, and a 48-kD form, p48TC (Champion-Arnaud et al., 1991; Mosinger et al., 1992). This manuscript concerns structural motifs in the noncatalytic segment of the enzyme responsible for targeting the two forms to different subcellular compartments. Endogenous and transiently expressed p48TC associates with the ER, as determined by sucrose gradient fractionation and indirect immunofluorescence, respectively. By contrast, p45TC localizes in the nucleus even though upon cell lysis it is not retained and fractionates with markers for soluble enzymes. Using fusion proteins consisting of beta-galactosidase and COOH- terminal fragments of p48TC, two motifs necessary for ER retention within a 70-residue targeting segment have been identified. These include the terminal 19 hydrophobic residues which comprise a potential membrane-spanning segment and residues 346-358 which encompass a cluster of basic amino acids that may represent another type of ER retention motif. The sequence RKRKR, which immediately precedes the splice junction, functions as a nuclear localization signal for p45TC.
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spelling pubmed-21206152008-05-01 COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus J Cell Biol Articles The noncatalytic domain of the human T cell protein tyrosine phosphatase (TCPTP) is alternatively spliced to generate a 45-kD form, p45TC, and a 48-kD form, p48TC (Champion-Arnaud et al., 1991; Mosinger et al., 1992). This manuscript concerns structural motifs in the noncatalytic segment of the enzyme responsible for targeting the two forms to different subcellular compartments. Endogenous and transiently expressed p48TC associates with the ER, as determined by sucrose gradient fractionation and indirect immunofluorescence, respectively. By contrast, p45TC localizes in the nucleus even though upon cell lysis it is not retained and fractionates with markers for soluble enzymes. Using fusion proteins consisting of beta-galactosidase and COOH- terminal fragments of p48TC, two motifs necessary for ER retention within a 70-residue targeting segment have been identified. These include the terminal 19 hydrophobic residues which comprise a potential membrane-spanning segment and residues 346-358 which encompass a cluster of basic amino acids that may represent another type of ER retention motif. The sequence RKRKR, which immediately precedes the splice junction, functions as a nuclear localization signal for p45TC. The Rockefeller University Press 1995-11-01 /pmc/articles/PMC2120615/ /pubmed/7593185 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus
title COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus
title_full COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus
title_fullStr COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus
title_full_unstemmed COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus
title_short COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus
title_sort cooh-terminal sequence motifs target the t cell protein tyrosine phosphatase to the er and nucleus
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120615/
https://www.ncbi.nlm.nih.gov/pubmed/7593185