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The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop

No targeting sequence for peroxisomal integral membrane proteins has yet been identified. We have previously shown that a region of 67 amino acids is necessary to target Pmp47, a protein that spans the membrane six times, to peroxisomes. This region comprises two membrane spans and the intervening l...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120804/
https://www.ncbi.nlm.nih.gov/pubmed/8609161
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collection PubMed
description No targeting sequence for peroxisomal integral membrane proteins has yet been identified. We have previously shown that a region of 67 amino acids is necessary to target Pmp47, a protein that spans the membrane six times, to peroxisomes. This region comprises two membrane spans and the intervening loop. We now demonstrate that the 20 amino acid loop, which is predicted to face the matrix, is both necessary and sufficient for peroxisomal targeting. Sufficiency was demonstrated with both chloramphenicol acetyltransferase and green fluorescent protein as carriers. There is a cluster of basic amino acids in the middle of the loop that we predict protrudes from the membrane surface into the matrix by a flanking stem structure. We show that the targeting signal is composed of this basic cluster and a block of amino acids immediately down-stream from it.
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spelling pubmed-21208042008-05-01 The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop J Cell Biol Articles No targeting sequence for peroxisomal integral membrane proteins has yet been identified. We have previously shown that a region of 67 amino acids is necessary to target Pmp47, a protein that spans the membrane six times, to peroxisomes. This region comprises two membrane spans and the intervening loop. We now demonstrate that the 20 amino acid loop, which is predicted to face the matrix, is both necessary and sufficient for peroxisomal targeting. Sufficiency was demonstrated with both chloramphenicol acetyltransferase and green fluorescent protein as carriers. There is a cluster of basic amino acids in the middle of the loop that we predict protrudes from the membrane surface into the matrix by a flanking stem structure. We show that the targeting signal is composed of this basic cluster and a block of amino acids immediately down-stream from it. The Rockefeller University Press 1996-04-02 /pmc/articles/PMC2120804/ /pubmed/8609161 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop
title The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop
title_full The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop
title_fullStr The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop
title_full_unstemmed The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop
title_short The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop
title_sort sorting sequence of the peroxisomal integral membrane protein pmp47 is contained within a short hydrophilic loop
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120804/
https://www.ncbi.nlm.nih.gov/pubmed/8609161