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Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei
We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. Approximately 10% of the U2 snRNP and an SR non-...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1996
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120859/ https://www.ncbi.nlm.nih.gov/pubmed/8655585 |
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collection | PubMed |
description | We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. Approximately 10% of the U2 snRNP and an SR non-snRNP splicing factor are associated with the chromosomes, highly enriched in active gene loci where they are bound to RNA. We demonstrate that the splicing factors are specifically recruited to a defined gene upon induction of transcription during physiological conditions. Concomitantly, the splicing factors leave gene loci in which transcription is turned off. We also demonstrated that upon general transcription inhibition, the splicing factors redistribute from active gene loci to the interchromatin. Our findings demonstrate the dynamic intranuclear organization of splicing factors and a tight linkage between transcription and the intranuclear organization of the splicing machinery. |
format | Text |
id | pubmed-2120859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1996 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21208592008-05-01 Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei J Cell Biol Articles We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. Approximately 10% of the U2 snRNP and an SR non-snRNP splicing factor are associated with the chromosomes, highly enriched in active gene loci where they are bound to RNA. We demonstrate that the splicing factors are specifically recruited to a defined gene upon induction of transcription during physiological conditions. Concomitantly, the splicing factors leave gene loci in which transcription is turned off. We also demonstrated that upon general transcription inhibition, the splicing factors redistribute from active gene loci to the interchromatin. Our findings demonstrate the dynamic intranuclear organization of splicing factors and a tight linkage between transcription and the intranuclear organization of the splicing machinery. The Rockefeller University Press 1996-06-01 /pmc/articles/PMC2120859/ /pubmed/8655585 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei |
title | Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei |
title_full | Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei |
title_fullStr | Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei |
title_full_unstemmed | Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei |
title_short | Demonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei |
title_sort | demonstration of a dynamic, transcription-dependent organization of pre- mrna splicing factors in polytene nuclei |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120859/ https://www.ncbi.nlm.nih.gov/pubmed/8655585 |