Sorting by COP I-coated vesicles under interphase and mitotic conditions

COP I-coated vesicles were analyzed for their content of resident Golgi enzymes (N-acetylgalactosaminyltransferase; N- acetylglucosaminyltransferase I; mannosidase II; galactosyltransferase), cargo (rat serum albumin; polyimmunoglobulin receptor), and recycling proteins (-KDEL receptor; ERGIC-53/p58...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1996
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120996/
https://www.ncbi.nlm.nih.gov/pubmed/8830771
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collection PubMed
description COP I-coated vesicles were analyzed for their content of resident Golgi enzymes (N-acetylgalactosaminyltransferase; N- acetylglucosaminyltransferase I; mannosidase II; galactosyltransferase), cargo (rat serum albumin; polyimmunoglobulin receptor), and recycling proteins (-KDEL receptor; ERGIC-53/p58) using biochemical and morphological techniques. The levels of these proteins were similar when the vesicles were prepared under interphase or mitotic conditions showing that sorting was unaffected. The average density relative to starting membranes for resident enzymes (14-30%), cargo (16-23%), and recycling proteins (81-125%) provides clues to the function of COP I vesicles in transport through the Golgi apparatus.
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spelling pubmed-21209962008-05-01 Sorting by COP I-coated vesicles under interphase and mitotic conditions J Cell Biol Articles COP I-coated vesicles were analyzed for their content of resident Golgi enzymes (N-acetylgalactosaminyltransferase; N- acetylglucosaminyltransferase I; mannosidase II; galactosyltransferase), cargo (rat serum albumin; polyimmunoglobulin receptor), and recycling proteins (-KDEL receptor; ERGIC-53/p58) using biochemical and morphological techniques. The levels of these proteins were similar when the vesicles were prepared under interphase or mitotic conditions showing that sorting was unaffected. The average density relative to starting membranes for resident enzymes (14-30%), cargo (16-23%), and recycling proteins (81-125%) provides clues to the function of COP I vesicles in transport through the Golgi apparatus. The Rockefeller University Press 1996-09-02 /pmc/articles/PMC2120996/ /pubmed/8830771 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Sorting by COP I-coated vesicles under interphase and mitotic conditions
title Sorting by COP I-coated vesicles under interphase and mitotic conditions
title_full Sorting by COP I-coated vesicles under interphase and mitotic conditions
title_fullStr Sorting by COP I-coated vesicles under interphase and mitotic conditions
title_full_unstemmed Sorting by COP I-coated vesicles under interphase and mitotic conditions
title_short Sorting by COP I-coated vesicles under interphase and mitotic conditions
title_sort sorting by cop i-coated vesicles under interphase and mitotic conditions
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120996/
https://www.ncbi.nlm.nih.gov/pubmed/8830771

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