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Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila
The wing of Drosophila melanogaster is covered by an array of distally pointing hairs. A hair begins as a single membrane outgrowth from each wing epithelial cell, and its distal orientation is determined by the restriction of outgrowth to a single distal site on the cell circumference (Wong, L., an...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1996
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2121092/ https://www.ncbi.nlm.nih.gov/pubmed/8947551 |
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collection | PubMed |
description | The wing of Drosophila melanogaster is covered by an array of distally pointing hairs. A hair begins as a single membrane outgrowth from each wing epithelial cell, and its distal orientation is determined by the restriction of outgrowth to a single distal site on the cell circumference (Wong, L., and P. Adler. 1993. J. Cell Biol. 123:209- 211.). We have examined the roles of Cdc42 and Rac1 in the formation of wing hairs. We find that Cdc42 is required for localized actin polymerization in the extending hair. Interfering with Cdc42 activity by expression of a dominant negative protein abolishes both localized actin polymerization and hair outgrowth. In contrast, Rac1 is important for restricting the site at which hairs grow out. Cells expressing the dominant negative Rac1N17 fail to restrict outgrowth to a single site and give rise to multiple wing hairs. This polarity defect is associated with disturbances in the organization of junctional actin and also with disruption of an intricate microtubule network that is intimately associated with the junctional region. We also find that apical junctions and microtubules are involved in structural aspects of hair outgrowth. During hair formation, the apical microtubules that point distally elongate and fill the emerging wing hair. As the hair elongates, junctional proteins are reorganized on the proximal and distal edges of each cell. |
format | Text |
id | pubmed-2121092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1996 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21210922008-05-01 Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila J Cell Biol Articles The wing of Drosophila melanogaster is covered by an array of distally pointing hairs. A hair begins as a single membrane outgrowth from each wing epithelial cell, and its distal orientation is determined by the restriction of outgrowth to a single distal site on the cell circumference (Wong, L., and P. Adler. 1993. J. Cell Biol. 123:209- 211.). We have examined the roles of Cdc42 and Rac1 in the formation of wing hairs. We find that Cdc42 is required for localized actin polymerization in the extending hair. Interfering with Cdc42 activity by expression of a dominant negative protein abolishes both localized actin polymerization and hair outgrowth. In contrast, Rac1 is important for restricting the site at which hairs grow out. Cells expressing the dominant negative Rac1N17 fail to restrict outgrowth to a single site and give rise to multiple wing hairs. This polarity defect is associated with disturbances in the organization of junctional actin and also with disruption of an intricate microtubule network that is intimately associated with the junctional region. We also find that apical junctions and microtubules are involved in structural aspects of hair outgrowth. During hair formation, the apical microtubules that point distally elongate and fill the emerging wing hair. As the hair elongates, junctional proteins are reorganized on the proximal and distal edges of each cell. The Rockefeller University Press 1996-12-01 /pmc/articles/PMC2121092/ /pubmed/8947551 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila |
title | Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila |
title_full | Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila |
title_fullStr | Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila |
title_full_unstemmed | Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila |
title_short | Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila |
title_sort | roles for rac1 and cdc42 in planar polarization and hair outgrowth in the wing of drosophila |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2121092/ https://www.ncbi.nlm.nih.gov/pubmed/8947551 |