Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli

BACKGROUND: Cloning of genes in expression libraries, such as the yeast two-hybrid system (Y2H), is based on the assumption that the loss of target genes is minimal, or at worst, managable. However, the expression of genes or gene fragments that are capable of interacting with E. coli or yeast gene...

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Detalles Bibliográficos
Autores principales: Haney, Steven A, Keeney, David, Chen, Lei, Moghazeh, Soraya, Projan, Steve, Rasmussen, Beth
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC212392/
https://www.ncbi.nlm.nih.gov/pubmed/12964949
http://dx.doi.org/10.1186/1471-2164-4-36

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