A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS

Individual, living, tissue cells can be obtained in suspension by digesting with trypsin the clot of growing tissue cultures. Under these circumstances the living cells assume a spherical form. When washed and plated in fresh plasma they put out processes and proliferate. After growth in the new pla...

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Detalles Bibliográficos
Autores principales: Rous, Peyton, Jones, F. S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1916
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2125431/
https://www.ncbi.nlm.nih.gov/pubmed/19868005
Descripción
Sumario:Individual, living, tissue cells can be obtained in suspension by digesting with trypsin the clot of growing tissue cultures. Under these circumstances the living cells assume a spherical form. When washed and plated in fresh plasma they put out processes and proliferate. After growth in the new plates has occurred the digestion and plating can be repeated. The limits of the method have not yet been reached. We are at work on a number of the problems which it has opened up.

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