PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY

A standardized method is described in detail by which collodion sacs suitable for intraperitoneal incubation and for other bacteriological experiments may be produced in large numbers, sterilized, and handled with convenience and the minimum danger of contamination. Various factors influencing perme...

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Detalles Bibliográficos
Autor principal: Gates, Frederick L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1921
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128168/
https://www.ncbi.nlm.nih.gov/pubmed/19868478
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author Gates, Frederick L.
author_facet Gates, Frederick L.
author_sort Gates, Frederick L.
collection PubMed
description A standardized method is described in detail by which collodion sacs suitable for intraperitoneal incubation and for other bacteriological experiments may be produced in large numbers, sterilized, and handled with convenience and the minimum danger of contamination. Various factors influencing permeability have been subjected to experiment. Like Brown, we found that immersion in alcohol is the most important factor, but the high permeability conferred by alcohol treatment is lost during heat sterilization if the membrane was previously allowed to dry. Quantitative experiments on the dialysis of sodium chloride, and simple tests with other substances indicate the general character of the membranes and their probable field of usefulness in bacteriology.
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spelling pubmed-21281682008-04-18 PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY Gates, Frederick L. J Exp Med Article A standardized method is described in detail by which collodion sacs suitable for intraperitoneal incubation and for other bacteriological experiments may be produced in large numbers, sterilized, and handled with convenience and the minimum danger of contamination. Various factors influencing permeability have been subjected to experiment. Like Brown, we found that immersion in alcohol is the most important factor, but the high permeability conferred by alcohol treatment is lost during heat sterilization if the membrane was previously allowed to dry. Quantitative experiments on the dialysis of sodium chloride, and simple tests with other substances indicate the general character of the membranes and their probable field of usefulness in bacteriology. The Rockefeller University Press 1921-01-01 /pmc/articles/PMC2128168/ /pubmed/19868478 Text en Copyright © Copyright, 1921, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gates, Frederick L.
PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY
title PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY
title_full PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY
title_fullStr PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY
title_full_unstemmed PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY
title_short PREPARATION OF COLLODION SACS FOR USE IN BACTERIOLOGY
title_sort preparation of collodion sacs for use in bacteriology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128168/
https://www.ncbi.nlm.nih.gov/pubmed/19868478
work_keys_str_mv AT gatesfrederickl preparationofcollodionsacsforuseinbacteriology

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