PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION

Antigens were prepared from the culture filtrates of tubercle bacilli and by extraction of washed and dried organisms with organic and aqueous solvents and from tissues of organs showing tuberculous lesions. A comparison of these preparations by means of the complement fixation test showed that the...

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Detalles Bibliográficos
Autores principales: Wadsworth, Augustus B., Maltaner, Frank
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1921
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128169/
https://www.ncbi.nlm.nih.gov/pubmed/19868476
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author Wadsworth, Augustus B.
Maltaner, Frank
author_facet Wadsworth, Augustus B.
Maltaner, Frank
author_sort Wadsworth, Augustus B.
collection PubMed
description Antigens were prepared from the culture filtrates of tubercle bacilli and by extraction of washed and dried organisms with organic and aqueous solvents and from tissues of organs showing tuberculous lesions. A comparison of these preparations by means of the complement fixation test showed that the aqueous extracts were most active antigenically. The antigenic activity of the aqueous preparations and also of the slightly active culture filtrates was increased by means of dialysis and adsorption with serum globulin followed by extraction with alcohol and concentration of the alcoholic extract in vacuo.
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spelling pubmed-21281692008-04-18 PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION Wadsworth, Augustus B. Maltaner, Frank J Exp Med Article Antigens were prepared from the culture filtrates of tubercle bacilli and by extraction of washed and dried organisms with organic and aqueous solvents and from tissues of organs showing tuberculous lesions. A comparison of these preparations by means of the complement fixation test showed that the aqueous extracts were most active antigenically. The antigenic activity of the aqueous preparations and also of the slightly active culture filtrates was increased by means of dialysis and adsorption with serum globulin followed by extraction with alcohol and concentration of the alcoholic extract in vacuo. The Rockefeller University Press 1921-01-01 /pmc/articles/PMC2128169/ /pubmed/19868476 Text en Copyright © Copyright, 1921, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wadsworth, Augustus B.
Maltaner, Frank
PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION
title PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION
title_full PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION
title_fullStr PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION
title_full_unstemmed PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION
title_short PURIFICATION AND CONCENTRATION OF ANTIGENS FOR COMPLEMENT FIXATION BY METHODS OF DIALYSIS, ADSORPTION, AND EXTRACTION
title_sort purification and concentration of antigens for complement fixation by methods of dialysis, adsorption, and extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128169/
https://www.ncbi.nlm.nih.gov/pubmed/19868476
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