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PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING

Attention is called to the fact that bacteria may be preserved for a long time by desiccation in the frozen state. It has been shown that it is necessary to maintain the frozen condition until desiccation is complete; if the fluid melts before the moisture is completely removed, the organisms are ki...

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Detalles Bibliográficos
Autor principal: Swift, Homer F.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1921
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128170/
https://www.ncbi.nlm.nih.gov/pubmed/19868481
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author Swift, Homer F.
author_facet Swift, Homer F.
author_sort Swift, Homer F.
collection PubMed
description Attention is called to the fact that bacteria may be preserved for a long time by desiccation in the frozen state. It has been shown that it is necessary to maintain the frozen condition until desiccation is complete; if the fluid melts before the moisture is completely removed, the organisms are killed, probably because of the concentration of the salts upon the surface of the bacteria. By the simple expedient of immersing the tubes of organisms in glycerol contained in a desiccator and subsequently keeping the whole apparatus in a salt-ice mixture until drying is complete, the organisms are easily maintained in the frozen state, and dry properly. Bacteria preserved in this manner retain their cultural, biochemical, and immunological characters for prolonged periods.
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spelling pubmed-21281702008-04-18 PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING Swift, Homer F. J Exp Med Article Attention is called to the fact that bacteria may be preserved for a long time by desiccation in the frozen state. It has been shown that it is necessary to maintain the frozen condition until desiccation is complete; if the fluid melts before the moisture is completely removed, the organisms are killed, probably because of the concentration of the salts upon the surface of the bacteria. By the simple expedient of immersing the tubes of organisms in glycerol contained in a desiccator and subsequently keeping the whole apparatus in a salt-ice mixture until drying is complete, the organisms are easily maintained in the frozen state, and dry properly. Bacteria preserved in this manner retain their cultural, biochemical, and immunological characters for prolonged periods. The Rockefeller University Press 1921-01-01 /pmc/articles/PMC2128170/ /pubmed/19868481 Text en Copyright © Copyright, 1921, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Swift, Homer F.
PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING
title PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING
title_full PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING
title_fullStr PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING
title_full_unstemmed PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING
title_short PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING
title_sort preservation of stock cultures of bacteria by freezing and drying
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128170/
https://www.ncbi.nlm.nih.gov/pubmed/19868481
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