STABLE SUSPENSIONS OF AUTOAGGLUTINABLE BACTERIA

Autoagglutinating cultures of Type G, bacillus of rabbit septicemia, can be rendered stable by suspension in glycocoll-acetate-phosphate buffer mixtures pH 7.5 and 7.1. Distilled water suspensions can also be employed if the normal and immune serum under test is diluted with buffers of this pH inste...

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Detalles Bibliográficos
Autores principales: De Kruif, Paul H., Northrop, John H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1923
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128352/
https://www.ncbi.nlm.nih.gov/pubmed/19868749
Descripción
Sumario:Autoagglutinating cultures of Type G, bacillus of rabbit septicemia, can be rendered stable by suspension in glycocoll-acetate-phosphate buffer mixtures pH 7.5 and 7.1. Distilled water suspensions can also be employed if the normal and immune serum under test is diluted with buffers of this pH instead of with 0.85 per cent NaCl. Five intensely autoagglutinable strains of Streptococcus hæmolyticus have been stabilized by washing and by suspending in 0.001 N NaOH. Specific agglutination of these stable suspensions by rabbit antistreptococcus serum could be demonstrated by diluting the serum with M/320 NaCl.

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