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SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION.
1. Pure lines of Spirochœta eurygyrata were isolated and cultured. 2. They lived longest in a medium which was made up of sodium chloride and pig serum water. 3. They stained readily with iron-hematoxylin, Giemsa's stain, Cross' stain, and carbolfuchsin. 4. They did not stain with the vita...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1922
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128391/ https://www.ncbi.nlm.nih.gov/pubmed/19868697 |
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author | Hogue, Mary Jane |
author_facet | Hogue, Mary Jane |
author_sort | Hogue, Mary Jane |
collection | PubMed |
description | 1. Pure lines of Spirochœta eurygyrata were isolated and cultured. 2. They lived longest in a medium which was made up of sodium chloride and pig serum water. 3. They stained readily with iron-hematoxylin, Giemsa's stain, Cross' stain, and carbolfuchsin. 4. They did not stain with the vital dyes used. 5. They divide by transverse division. 6. Transverse bars, which are seen in stained spirochetes and in dead ones with dark-field illumination, are considered coagulated protoplasm, due to death. 7. Coccoid bodies were not seen in living individuals nor were they seen free in the culture medium. 8. Cats were fed cultures of Spirochœta eurygyrata but did not become infected with them. 9. Spirochœta eurygyrata is not of frequent occurrence in this part of the United States. |
format | Text |
id | pubmed-2128391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1922 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21283912008-04-18 SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION. Hogue, Mary Jane J Exp Med Article 1. Pure lines of Spirochœta eurygyrata were isolated and cultured. 2. They lived longest in a medium which was made up of sodium chloride and pig serum water. 3. They stained readily with iron-hematoxylin, Giemsa's stain, Cross' stain, and carbolfuchsin. 4. They did not stain with the vital dyes used. 5. They divide by transverse division. 6. Transverse bars, which are seen in stained spirochetes and in dead ones with dark-field illumination, are considered coagulated protoplasm, due to death. 7. Coccoid bodies were not seen in living individuals nor were they seen free in the culture medium. 8. Cats were fed cultures of Spirochœta eurygyrata but did not become infected with them. 9. Spirochœta eurygyrata is not of frequent occurrence in this part of the United States. The Rockefeller University Press 1922-11-30 /pmc/articles/PMC2128391/ /pubmed/19868697 Text en Copyright © Copyright, 1922, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Hogue, Mary Jane SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION. |
title | SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION. |
title_full | SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION. |
title_fullStr | SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION. |
title_full_unstemmed | SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION. |
title_short | SPIROCHÆTA EURYGYRATA : A NOTE ON ITS LIFE HISTORY AND CULTIVATION. |
title_sort | spirochæta eurygyrata : a note on its life history and cultivation. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2128391/ https://www.ncbi.nlm.nih.gov/pubmed/19868697 |
work_keys_str_mv | AT hoguemaryjane spirochætaeurygyrataanoteonitslifehistoryandcultivation |