Cargando…

THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.

The acidity of the macrophage granules in which litmus comes to be stored during life is considerable. It has proved possible to stain these granules in vivo with some of the phthalein indicators and the results, had they been obtained under controlled conditions, would indicate a pH of 3.0 or less....

Descripción completa

Detalles Bibliográficos
Autor principal: Rous, Peyton
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1925
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2130947/
https://www.ncbi.nlm.nih.gov/pubmed/19868996
_version_ 1782142134964453376
author Rous, Peyton
author_facet Rous, Peyton
author_sort Rous, Peyton
collection PubMed
description The acidity of the macrophage granules in which litmus comes to be stored during life is considerable. It has proved possible to stain these granules in vivo with some of the phthalein indicators and the results, had they been obtained under controlled conditions, would indicate a pH of 3.0 or less. The amount of acid material which may accumulate within the cells of animals stained with litmus is great, sufficient in the case of the elements of a peritoneal exudate for the acid reaction to prevail when they are gathered together and crushed. The material is derived, not from the dye, but from living elements responding characteristically to a stimulus far from unique. Such responses may well play a rôle in normal physiological activities and in the cellular defense against microorganisms. Vital staining with litmus demonstrates anew that the intracellular reaction during life is independent of that of the body fluids. By means of color changes in the stored indicator one can distinguish sick as well as dead cells of certain sorts and follow their distribution and fate within the organism. There are data to suggest that with the aid of the indicator the normal period of survival of certain elements at least can be determined. By the indicator method, of which the foregoing observations afford a crude illustration, much should be learnt in the future about body processes. The present paper is the second of a series upon the theme.
format Text
id pubmed-2130947
institution National Center for Biotechnology Information
language English
publishDate 1925
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21309472008-04-18 THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE. Rous, Peyton J Exp Med Article The acidity of the macrophage granules in which litmus comes to be stored during life is considerable. It has proved possible to stain these granules in vivo with some of the phthalein indicators and the results, had they been obtained under controlled conditions, would indicate a pH of 3.0 or less. The amount of acid material which may accumulate within the cells of animals stained with litmus is great, sufficient in the case of the elements of a peritoneal exudate for the acid reaction to prevail when they are gathered together and crushed. The material is derived, not from the dye, but from living elements responding characteristically to a stimulus far from unique. Such responses may well play a rôle in normal physiological activities and in the cellular defense against microorganisms. Vital staining with litmus demonstrates anew that the intracellular reaction during life is independent of that of the body fluids. By means of color changes in the stored indicator one can distinguish sick as well as dead cells of certain sorts and follow their distribution and fate within the organism. There are data to suggest that with the aid of the indicator the normal period of survival of certain elements at least can be determined. By the indicator method, of which the foregoing observations afford a crude illustration, much should be learnt in the future about body processes. The present paper is the second of a series upon the theme. The Rockefeller University Press 1925-02-28 /pmc/articles/PMC2130947/ /pubmed/19868996 Text en Copyright © Copyright, 1925, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rous, Peyton
THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.
title THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.
title_full THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.
title_fullStr THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.
title_full_unstemmed THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.
title_short THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES : II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.
title_sort relative reaction within living mammalian-tissues : ii. on the mobilization of acid material within cells, and the reaction as influenced by the cell state.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2130947/
https://www.ncbi.nlm.nih.gov/pubmed/19868996
work_keys_str_mv AT rouspeyton therelativereactionwithinlivingmammaliantissuesiionthemobilizationofacidmaterialwithincellsandthereactionasinfluencedbythecellstate
AT rouspeyton relativereactionwithinlivingmammaliantissuesiionthemobilizationofacidmaterialwithincellsandthereactionasinfluencedbythecellstate