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A STUDY OF PNEUMOCOCCUS AUTOLYSIS

1. Autolysis of Pneumococcus is accompanied by proteolysis, which results in an increase in amino and non-coagulable nitrogen. 2. Autolysis of Pneumococcus is accompanied by lipolysis during which there is a liberation of ether-soluble fatty acids. 3. When extracts containing the active intracellula...

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Autores principales: Goebel, Walther F., Avery, Oswald T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1929
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131532/
https://www.ncbi.nlm.nih.gov/pubmed/19869547
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author Goebel, Walther F.
Avery, Oswald T.
author_facet Goebel, Walther F.
Avery, Oswald T.
author_sort Goebel, Walther F.
collection PubMed
description 1. Autolysis of Pneumococcus is accompanied by proteolysis, which results in an increase in amino and non-coagulable nitrogen. 2. Autolysis of Pneumococcus is accompanied by lipolysis during which there is a liberation of ether-soluble fatty acids. 3. When extracts containing the active intracellular enzymes are added to heat-killed pneumococci, lysis of the cells occurs and there is an increase in the non-coagulable and amino nitrogen, comparable to the changes accompanying spontaneous autolysis. 4. When extracts containing the active intracellular enzymes are added to emulsions of the alcohol-soluble lipoids extracted from pneumococci, an increase in the ether-soluble fatty acid occurs. 5. Sodium desoxycholate in excess inhibits the action of pneumococcus protease; it does not inhibit the action of pneumococcus lipase. 6. When suspensions of pneumococci are cooled to 0°C., a temperature at which the rate of enzyme action is greatly retarded, the organisms go into solution rapidly when sodium desoxycholate is added, but this process is not accompanied by lipolysis or proteolysis. It does not seem probable, therefore, that the "bile" solution of pneumococci is identical with the phenomenon of autolysis as ordinarily understood and measured. 7. The relation of enzyme action to antigenic dissociation is discussed.
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spelling pubmed-21315322008-04-18 A STUDY OF PNEUMOCOCCUS AUTOLYSIS Goebel, Walther F. Avery, Oswald T. J Exp Med Article 1. Autolysis of Pneumococcus is accompanied by proteolysis, which results in an increase in amino and non-coagulable nitrogen. 2. Autolysis of Pneumococcus is accompanied by lipolysis during which there is a liberation of ether-soluble fatty acids. 3. When extracts containing the active intracellular enzymes are added to heat-killed pneumococci, lysis of the cells occurs and there is an increase in the non-coagulable and amino nitrogen, comparable to the changes accompanying spontaneous autolysis. 4. When extracts containing the active intracellular enzymes are added to emulsions of the alcohol-soluble lipoids extracted from pneumococci, an increase in the ether-soluble fatty acid occurs. 5. Sodium desoxycholate in excess inhibits the action of pneumococcus protease; it does not inhibit the action of pneumococcus lipase. 6. When suspensions of pneumococci are cooled to 0°C., a temperature at which the rate of enzyme action is greatly retarded, the organisms go into solution rapidly when sodium desoxycholate is added, but this process is not accompanied by lipolysis or proteolysis. It does not seem probable, therefore, that the "bile" solution of pneumococci is identical with the phenomenon of autolysis as ordinarily understood and measured. 7. The relation of enzyme action to antigenic dissociation is discussed. The Rockefeller University Press 1929-01-31 /pmc/articles/PMC2131532/ /pubmed/19869547 Text en Copyright © Copyright, 1929, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Goebel, Walther F.
Avery, Oswald T.
A STUDY OF PNEUMOCOCCUS AUTOLYSIS
title A STUDY OF PNEUMOCOCCUS AUTOLYSIS
title_full A STUDY OF PNEUMOCOCCUS AUTOLYSIS
title_fullStr A STUDY OF PNEUMOCOCCUS AUTOLYSIS
title_full_unstemmed A STUDY OF PNEUMOCOCCUS AUTOLYSIS
title_short A STUDY OF PNEUMOCOCCUS AUTOLYSIS
title_sort study of pneumococcus autolysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131532/
https://www.ncbi.nlm.nih.gov/pubmed/19869547
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