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Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation

The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is es...

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Detalles Bibliográficos
Autores principales: Brittle, Amy L., Nanba, Yasuaki, Ito, Takashi, Ohkura, Hiroyuki
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131725/
https://www.ncbi.nlm.nih.gov/pubmed/17586492
http://dx.doi.org/10.1016/j.yexcr.2007.04.038
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author Brittle, Amy L.
Nanba, Yasuaki
Ito, Takashi
Ohkura, Hiroyuki
author_facet Brittle, Amy L.
Nanba, Yasuaki
Ito, Takashi
Ohkura, Hiroyuki
author_sort Brittle, Amy L.
collection PubMed
description The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis. Cyclin B degradation triggers loss of centromeric H2A phosphorylation at anaphase onset. Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B. Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation.
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spelling pubmed-21317252007-12-13 Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation Brittle, Amy L. Nanba, Yasuaki Ito, Takashi Ohkura, Hiroyuki Exp Cell Res Research Article The spatial and temporal control of histone modifications is crucial for precise regulation of chromatin structure and function. Here we report that phosphorylation of H2A at threonine 119 (T119) is enriched at centromere regions in Drosophila mitosis. We found that the Aurora B kinase complex is essential for this phosphorylation at centromeres, while Polo kinase is required to down-regulate H2A phosphorylation on chromosome arms in mitosis. Cyclin B degradation triggers loss of centromeric H2A phosphorylation at anaphase onset. Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B. Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation. Academic Press 2007-05-25 /pmc/articles/PMC2131725/ /pubmed/17586492 http://dx.doi.org/10.1016/j.yexcr.2007.04.038 Text en . https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Research Article
Brittle, Amy L.
Nanba, Yasuaki
Ito, Takashi
Ohkura, Hiroyuki
Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation
title Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation
title_full Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation
title_fullStr Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation
title_full_unstemmed Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation
title_short Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation
title_sort concerted action of aurora b, polo and nhk-1 kinases in centromere-specific histone 2a phosphorylation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131725/
https://www.ncbi.nlm.nih.gov/pubmed/17586492
http://dx.doi.org/10.1016/j.yexcr.2007.04.038
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