A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS

A simple, rapid method for staining all the supravitally stainable cells in the body, as in supravital preparations with neutral red, is described, together with a method for faithful preservation of the dye in paraffin sections. The essential points of the technique are, first, to secure the reacti...

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Detalles Bibliográficos
Autor principal: Forkner, Claude E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1930
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131880/
https://www.ncbi.nlm.nih.gov/pubmed/19869771
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author Forkner, Claude E.
author_facet Forkner, Claude E.
author_sort Forkner, Claude E.
collection PubMed
description A simple, rapid method for staining all the supravitally stainable cells in the body, as in supravital preparations with neutral red, is described, together with a method for faithful preservation of the dye in paraffin sections. The essential points of the technique are, first, to secure the reaction of cells to neutral red which corresponds to the so-called supravital technique, involving the reaction of only those substances which respond to the dye while the cell is living; and second, to preserve the stain through the processes of fixation, embedding, and counterstaining.
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spelling pubmed-21318802008-04-18 A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS Forkner, Claude E. J Exp Med Article A simple, rapid method for staining all the supravitally stainable cells in the body, as in supravital preparations with neutral red, is described, together with a method for faithful preservation of the dye in paraffin sections. The essential points of the technique are, first, to secure the reaction of cells to neutral red which corresponds to the so-called supravital technique, involving the reaction of only those substances which respond to the dye while the cell is living; and second, to preserve the stain through the processes of fixation, embedding, and counterstaining. The Rockefeller University Press 1930-08-31 /pmc/articles/PMC2131880/ /pubmed/19869771 Text en Copyright © Copyright, 1930, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Forkner, Claude E.
A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS
title A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS
title_full A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS
title_fullStr A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS
title_full_unstemmed A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS
title_short A METHOD FOR SUPRAVITAL STAINING OF ANIMALS WITH NEUTRAL RED AND ITS PRESERVATION IN PARAFFIN SECTIONS
title_sort method for supravital staining of animals with neutral red and its preservation in paraffin sections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131880/
https://www.ncbi.nlm.nih.gov/pubmed/19869771
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