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Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus
Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and im...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132479/ https://www.ncbi.nlm.nih.gov/pubmed/9060466 |
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author | Sodeik, Beate Ebersold, Melanie W. Helenius, Ari |
author_facet | Sodeik, Beate Ebersold, Melanie W. Helenius, Ari |
author_sort | Sodeik, Beate |
collection | PubMed |
description | Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end–directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus. |
format | Text |
id | pubmed-2132479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21324792008-05-01 Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus Sodeik, Beate Ebersold, Melanie W. Helenius, Ari J Cell Biol Article Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end–directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus. The Rockefeller University Press 1997-03-10 /pmc/articles/PMC2132479/ /pubmed/9060466 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Sodeik, Beate Ebersold, Melanie W. Helenius, Ari Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus |
title | Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus |
title_full | Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus |
title_fullStr | Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus |
title_full_unstemmed | Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus |
title_short | Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus |
title_sort | microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132479/ https://www.ncbi.nlm.nih.gov/pubmed/9060466 |
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