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Conservation of the Centromere/Kinetochore Protein ZW10

Mutations in the essential Drosophila melanogaster gene zw10 disrupt chromosome segregation, producing chromosomes that lag at the metaphase plate during anaphase of mitosis and both meiotic divisions. Recent evidence suggests that the product of this gene, DmZW10, acts at the kinetochore as part of...

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Autores principales: Starr, Daniel A., Williams, Byron C., Li, Zexiao, Etemad-Moghadam, Bijan, Dawe, R. Kelly, Goldberg, Michael L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132553/
https://www.ncbi.nlm.nih.gov/pubmed/9298984
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author Starr, Daniel A.
Williams, Byron C.
Li, Zexiao
Etemad-Moghadam, Bijan
Dawe, R. Kelly
Goldberg, Michael L.
author_facet Starr, Daniel A.
Williams, Byron C.
Li, Zexiao
Etemad-Moghadam, Bijan
Dawe, R. Kelly
Goldberg, Michael L.
author_sort Starr, Daniel A.
collection PubMed
description Mutations in the essential Drosophila melanogaster gene zw10 disrupt chromosome segregation, producing chromosomes that lag at the metaphase plate during anaphase of mitosis and both meiotic divisions. Recent evidence suggests that the product of this gene, DmZW10, acts at the kinetochore as part of a tension-sensing checkpoint at anaphase onset. DmZW10 displays an intriguing cell cycle–dependent intracellular distribution, apparently moving from the centromere/kinetochore at prometaphase to kinetochore microtubules at metaphase, and back to the centromere/kinetochore at anaphase (Williams, B.C., M. Gatti, and M.L. Goldberg. 1996. J. Cell Biol. 134:1127-1140). We have identified ZW10-related proteins from widely diverse species with divergent centromere structures, including several Drosophilids, Caenorhabditis elegans, Arabidopsis thaliana, Mus musculus, and humans. Antibodies against the human ZW10 protein display a cell cycle–dependent staining pattern in HeLa cells strikingly similar to that previously observed for DmZW10 in dividing Drosophila cells. Injections of C. elegans ZW10 antisense RNA phenocopies important aspects of the mutant phenotype in Drosophila: these include a strong decrease in brood size, suggesting defects in meiosis or germline mitosis, a high percentage of lethality among the embryos that are produced, and the appearance of chromatin bridges at anaphase. These results indicate that at least some aspects of the functional role of the ZW10 protein in ensuring proper chromosome segregation are conserved across large evolutionary distances.
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spelling pubmed-21325532008-05-01 Conservation of the Centromere/Kinetochore Protein ZW10 Starr, Daniel A. Williams, Byron C. Li, Zexiao Etemad-Moghadam, Bijan Dawe, R. Kelly Goldberg, Michael L. J Cell Biol Article Mutations in the essential Drosophila melanogaster gene zw10 disrupt chromosome segregation, producing chromosomes that lag at the metaphase plate during anaphase of mitosis and both meiotic divisions. Recent evidence suggests that the product of this gene, DmZW10, acts at the kinetochore as part of a tension-sensing checkpoint at anaphase onset. DmZW10 displays an intriguing cell cycle–dependent intracellular distribution, apparently moving from the centromere/kinetochore at prometaphase to kinetochore microtubules at metaphase, and back to the centromere/kinetochore at anaphase (Williams, B.C., M. Gatti, and M.L. Goldberg. 1996. J. Cell Biol. 134:1127-1140). We have identified ZW10-related proteins from widely diverse species with divergent centromere structures, including several Drosophilids, Caenorhabditis elegans, Arabidopsis thaliana, Mus musculus, and humans. Antibodies against the human ZW10 protein display a cell cycle–dependent staining pattern in HeLa cells strikingly similar to that previously observed for DmZW10 in dividing Drosophila cells. Injections of C. elegans ZW10 antisense RNA phenocopies important aspects of the mutant phenotype in Drosophila: these include a strong decrease in brood size, suggesting defects in meiosis or germline mitosis, a high percentage of lethality among the embryos that are produced, and the appearance of chromatin bridges at anaphase. These results indicate that at least some aspects of the functional role of the ZW10 protein in ensuring proper chromosome segregation are conserved across large evolutionary distances. The Rockefeller University Press 1997-09-22 /pmc/articles/PMC2132553/ /pubmed/9298984 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Starr, Daniel A.
Williams, Byron C.
Li, Zexiao
Etemad-Moghadam, Bijan
Dawe, R. Kelly
Goldberg, Michael L.
Conservation of the Centromere/Kinetochore Protein ZW10
title Conservation of the Centromere/Kinetochore Protein ZW10
title_full Conservation of the Centromere/Kinetochore Protein ZW10
title_fullStr Conservation of the Centromere/Kinetochore Protein ZW10
title_full_unstemmed Conservation of the Centromere/Kinetochore Protein ZW10
title_short Conservation of the Centromere/Kinetochore Protein ZW10
title_sort conservation of the centromere/kinetochore protein zw10
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132553/
https://www.ncbi.nlm.nih.gov/pubmed/9298984
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