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Sarcomeric Gene Expression and Contractility in Myofibroblasts

Myofibroblasts are unusual cells that share morphological and functional features of muscle and nonmuscle cells. Such cells are thought to control liver blood flow and kidney glomerular filtration rate by having unique contractile properties. To determine how these cells achieve their contractile pr...

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Detalles Bibliográficos
Autores principales: Mayer, D.C. Ghislaine, Leinwand, Leslie A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132619/
https://www.ncbi.nlm.nih.gov/pubmed/9396753
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author Mayer, D.C. Ghislaine
Leinwand, Leslie A.
author_facet Mayer, D.C. Ghislaine
Leinwand, Leslie A.
author_sort Mayer, D.C. Ghislaine
collection PubMed
description Myofibroblasts are unusual cells that share morphological and functional features of muscle and nonmuscle cells. Such cells are thought to control liver blood flow and kidney glomerular filtration rate by having unique contractile properties. To determine how these cells achieve their contractile properties and their resemblance to muscle cells, we have characterized two myofibroblast cell lines. Here, we demonstrate that myofibroblast cell lines from kidney mesangial cells (BHK) and liver stellate cells activate extensive programs of muscle gene expression including a wide variety of muscle structural proteins. In BHK cells, six different striated myosin heavy chain isoforms and many thin filament proteins, including troponin T and tropomyosin are expressed. Liver stellate cells express a limited subset of the muscle thick filament proteins expressed in BHK cells. Although these cells are mitotically active and do not morphologically differentiate into myotubes, we show that MyoD and myogenin are expressed and functional in both cell types. Finally, these cells contract in response to endothelin-1 (ET-1); and we show that ET-1 treatment increases the expression of sarcomeric myosin.
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spelling pubmed-21326192008-05-01 Sarcomeric Gene Expression and Contractility in Myofibroblasts Mayer, D.C. Ghislaine Leinwand, Leslie A. J Cell Biol Article Myofibroblasts are unusual cells that share morphological and functional features of muscle and nonmuscle cells. Such cells are thought to control liver blood flow and kidney glomerular filtration rate by having unique contractile properties. To determine how these cells achieve their contractile properties and their resemblance to muscle cells, we have characterized two myofibroblast cell lines. Here, we demonstrate that myofibroblast cell lines from kidney mesangial cells (BHK) and liver stellate cells activate extensive programs of muscle gene expression including a wide variety of muscle structural proteins. In BHK cells, six different striated myosin heavy chain isoforms and many thin filament proteins, including troponin T and tropomyosin are expressed. Liver stellate cells express a limited subset of the muscle thick filament proteins expressed in BHK cells. Although these cells are mitotically active and do not morphologically differentiate into myotubes, we show that MyoD and myogenin are expressed and functional in both cell types. Finally, these cells contract in response to endothelin-1 (ET-1); and we show that ET-1 treatment increases the expression of sarcomeric myosin. The Rockefeller University Press 1997-12-15 /pmc/articles/PMC2132619/ /pubmed/9396753 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Mayer, D.C. Ghislaine
Leinwand, Leslie A.
Sarcomeric Gene Expression and Contractility in Myofibroblasts
title Sarcomeric Gene Expression and Contractility in Myofibroblasts
title_full Sarcomeric Gene Expression and Contractility in Myofibroblasts
title_fullStr Sarcomeric Gene Expression and Contractility in Myofibroblasts
title_full_unstemmed Sarcomeric Gene Expression and Contractility in Myofibroblasts
title_short Sarcomeric Gene Expression and Contractility in Myofibroblasts
title_sort sarcomeric gene expression and contractility in myofibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132619/
https://www.ncbi.nlm.nih.gov/pubmed/9396753
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