Cargando…

A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis

The molecular mechanisms regulating the spectacular cytodifferentiation observed during spermiogenesis are poorly understood. We have recently identified a murine testis-specific serine kinase (tssk) 1, constituting a novel subfamily of serine/threonine kinases. Using low stringency screening we hav...

Descripción completa

Detalles Bibliográficos
Autores principales: Kueng, Peter, Nikolova, Zariana, Djonov, Valentin, Hemphill, Andrew, Rohrbach, Valeria, Boehlen, Dominik, Zuercher, Gisela, Andres, Anne-Catherine, Ziemiecki, Andrew
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132653/
https://www.ncbi.nlm.nih.gov/pubmed/9412477
_version_ 1782142494785404928
author Kueng, Peter
Nikolova, Zariana
Djonov, Valentin
Hemphill, Andrew
Rohrbach, Valeria
Boehlen, Dominik
Zuercher, Gisela
Andres, Anne-Catherine
Ziemiecki, Andrew
author_facet Kueng, Peter
Nikolova, Zariana
Djonov, Valentin
Hemphill, Andrew
Rohrbach, Valeria
Boehlen, Dominik
Zuercher, Gisela
Andres, Anne-Catherine
Ziemiecki, Andrew
author_sort Kueng, Peter
collection PubMed
description The molecular mechanisms regulating the spectacular cytodifferentiation observed during spermiogenesis are poorly understood. We have recently identified a murine testis-specific serine kinase (tssk) 1, constituting a novel subfamily of serine/threonine kinases. Using low stringency screening we have isolated and molecularly characterized a second closely related family member, tssk 2, which is probably the orthologue of the human DGS-G gene. Expression of tssk 1 and tssk 2 was limited to the testis of sexually mature males. Immunohistochemical staining localized both kinases to the cytoplasm of late spermatids and to structures resembling residual bodies. tssk 1 and tssk 2 were absent in released sperms in the lumen of the seminiferous tubules and the epididymis, demonstrating a tight window of expression restricted to the last stages of spermatid maturation. In vitro kinase assays of immunoprecipitates containing either tssk 1 or tssk 2 revealed no autophosphorylation of the kinases, however, they led to serine phosphorylation of a coprecipitating protein of ∼65 kD. A search for interacting proteins using the yeast two-hybrid system with tssk 1 and tssk 2 cDNA as baits and a prey cDNA library from mouse testis, led to the isolation of a novel cDNA, interacting specifically with both tssk 1 and tssk 2, and encoding the coprecipitated 65-kD protein phosphorylated by both kinases. Interestingly, expression of the interacting clone was also testis specific and paralleled the developmental expression observed for the kinases themselves. These results represent the first demonstration of the involvement of a distinct kinase family, the tssk serine/threonine kinases, together with a substrate in the cytodifferentiation of late spermatids to sperms.
format Text
id pubmed-2132653
institution National Center for Biotechnology Information
language English
publishDate 1997
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21326532008-05-01 A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis Kueng, Peter Nikolova, Zariana Djonov, Valentin Hemphill, Andrew Rohrbach, Valeria Boehlen, Dominik Zuercher, Gisela Andres, Anne-Catherine Ziemiecki, Andrew J Cell Biol Article The molecular mechanisms regulating the spectacular cytodifferentiation observed during spermiogenesis are poorly understood. We have recently identified a murine testis-specific serine kinase (tssk) 1, constituting a novel subfamily of serine/threonine kinases. Using low stringency screening we have isolated and molecularly characterized a second closely related family member, tssk 2, which is probably the orthologue of the human DGS-G gene. Expression of tssk 1 and tssk 2 was limited to the testis of sexually mature males. Immunohistochemical staining localized both kinases to the cytoplasm of late spermatids and to structures resembling residual bodies. tssk 1 and tssk 2 were absent in released sperms in the lumen of the seminiferous tubules and the epididymis, demonstrating a tight window of expression restricted to the last stages of spermatid maturation. In vitro kinase assays of immunoprecipitates containing either tssk 1 or tssk 2 revealed no autophosphorylation of the kinases, however, they led to serine phosphorylation of a coprecipitating protein of ∼65 kD. A search for interacting proteins using the yeast two-hybrid system with tssk 1 and tssk 2 cDNA as baits and a prey cDNA library from mouse testis, led to the isolation of a novel cDNA, interacting specifically with both tssk 1 and tssk 2, and encoding the coprecipitated 65-kD protein phosphorylated by both kinases. Interestingly, expression of the interacting clone was also testis specific and paralleled the developmental expression observed for the kinases themselves. These results represent the first demonstration of the involvement of a distinct kinase family, the tssk serine/threonine kinases, together with a substrate in the cytodifferentiation of late spermatids to sperms. The Rockefeller University Press 1997-12-29 /pmc/articles/PMC2132653/ /pubmed/9412477 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kueng, Peter
Nikolova, Zariana
Djonov, Valentin
Hemphill, Andrew
Rohrbach, Valeria
Boehlen, Dominik
Zuercher, Gisela
Andres, Anne-Catherine
Ziemiecki, Andrew
A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis
title A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis
title_full A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis
title_fullStr A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis
title_full_unstemmed A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis
title_short A Novel Family of Serine/Threonine Kinases Participating in Spermiogenesis
title_sort novel family of serine/threonine kinases participating in spermiogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132653/
https://www.ncbi.nlm.nih.gov/pubmed/9412477
work_keys_str_mv AT kuengpeter anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT nikolovazariana anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT djonovvalentin anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT hemphillandrew anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT rohrbachvaleria anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT boehlendominik anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT zuerchergisela anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT andresannecatherine anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT ziemieckiandrew anovelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT kuengpeter novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT nikolovazariana novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT djonovvalentin novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT hemphillandrew novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT rohrbachvaleria novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT boehlendominik novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT zuerchergisela novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT andresannecatherine novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis
AT ziemieckiandrew novelfamilyofserinethreoninekinasesparticipatinginspermiogenesis