Cargando…

Functional Imaging of Mitochondria in Saponin-permeabilized Mice Muscle Fibers

Confocal laser-scanning and digital fluorescence imaging microscopy were used to quantify the mitochondrial autofluorescence changes of NAD(P)H and flavoproteins in unfixed saponin-permeabilized myofibers from mice quadriceps muscle tissue. Addition of mitochondrial substrates, ADP, or cyanide led t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kuznetsov, Andrey V., Mayboroda, Oleg, Kunz, Dagmar, Winkler, Kirstin, Schubert, Walter, Kunz, Wolfram S.
Formato:	Texto
Lenguaje:	English
Publicado:	The Rockefeller University Press 1998
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132706/ https://www.ncbi.nlm.nih.gov/pubmed/9490722

Ejemplares similares

Saponin-permeabilization is not a viable alternative to isolated mitochondria for assessing oxidative metabolism in hibernation
por: Mathers, Katherine E., et al.
Publicado: (2015)

Resistance of Dictyostelium discoideum membranes to saponin permeabilization
por: Mercanti, Valentina, et al.
Publicado: (2010)

Formation and contraction of a microfilamentous shell in saponin- permeabilized platelets
Publicado: (1991)

Direct measurement of Ca2+ uptake and release by the sarcoplasmic reticulum of saponin permeabilized isolated smooth muscle cells
Publicado: (1995)

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers
por: Roche, Stuart M., et al.
Publicado: (2015)

Coronavirus IBV glycopolypeptides: locational studies using proteases and saponin, a membrane permeabilizer
por: Cavanagh, David, et al.
Publicado: (1986)

Apparent Km of mitochondria for oxygen computed from Vmax measured in permeabilized muscle fibers is lower in water enriched in oxygen by electrolysis than injection
por: Zoll, Joffrey, et al.
Publicado: (2015)

Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria
por: Yang, Krista, et al.
Publicado: (2021)

Revisiting specific force loss in human permeabilized single skeletal muscle fibers obtained from older individuals
por: Kalakoutis, Michaeljohn, et al.
Publicado: (2023)

Replication fork rescue in mammalian mitochondria
por: Torregrosa-Muñumer, Rubén, et al.
Publicado: (2019)

Bcl-2 proteins bid and bax form a network to permeabilize the mitochondria at the onset of apoptosis
por: Gahl, Robert F, et al.
Publicado: (2016)

Gasdermin pores permeabilize mitochondria to augment caspase-3 activation during apoptosis and inflammasome activation
por: Rogers, Corey, et al.
Publicado: (2019)

Analysis of Mitochondrial Calcium Retention Capacity in Cultured Cells: Permeabilized Cells Versus Isolated Mitochondria
por: Jang, Sehwan, et al.
Publicado: (2021)

Permeabilized microbes in biotechnology
por: Wackett, Lawrence P
Publicado: (2014)

Editorial: Ion Transport in Chloroplast and Mitochondria Physiology in Green Organisms
por: Spetea, Cornelia, et al.
Publicado: (2017)

Non-cross Bridge Viscoelastic Elements Contribute to Muscle Force and Work During Stretch-Shortening Cycles: Evidence From Whole Muscles and Permeabilized Fibers
por: Hessel, Anthony L., et al.
Publicado: (2021)

Mitochondria: the cell powerhouse and nexus of stress
por: Javadov, Sabzali, et al.
Publicado: (2013)

Prevention Effect of Protopanaxadiol-Type Saponins Saponins and Protopanaxatriol-Type Saponins on Myelosuppression Mice Induced by Cyclophosphamide
por: Zhang, He, et al.
Publicado: (2022)

Calcium Ions Regulate K(+) Uptake into Brain Mitochondria: The Evidence for a Novel Potassium Channel
por: Skalska, Jolanta, et al.
Publicado: (2009)

High-Resolution Respirometry for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Fluxes in Permeabilized Cells, Tissue Homogenate and Isolated Mitochondria
por: Makrecka-Kuka, Marina, et al.
Publicado: (2015)

Changes of lysosomal membrane permeabilization and lipid metabolism in sidt2 deficient mice
por: Meng, Yu, et al.
Publicado: (2018)

Mechanisms of light‐induced liposome permeabilization
por: Miranda, Dyego, et al.
Publicado: (2016)

Permeabilizing Cell Membranes with Electric Fields
por: Aguilar, Alondra A., et al.
Publicado: (2021)

An Effective Chemical Permeabilization of Silkworm Embryos
por: Urbán-Duarte, David, et al.
Publicado: (2023)

Dystrophic Changes in Extraocular Muscles after Gamma Irradiation in mdx:utrophin(+/−) Mice
por: McDonald, Abby A., et al.
Publicado: (2014)

Diffusion Restrictions Surrounding Mitochondria: A Mathematical Model of Heart Muscle Fibers
por: Ramay, Hena R., et al.
Publicado: (2009)

Heterogeneity of Mitochondria and Mitochondrial Function within Cells as Another Level of Mitochondrial Complexity
por: Kuznetsov, Andrey V, et al.
Publicado: (2009)

Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)
por: Pereira, Diogo A., et al.
Publicado: (2021)

Use of Human Cancer Cell Lines Mitochondria to Explore the Mechanisms of BH3 Peptides and ABT-737-Induced Mitochondrial Membrane Permeabilization
por: Buron, Nelly, et al.
Publicado: (2010)

Metabolic Epilepsies—Commemorative Issue in Honor of Professor Uwe Heinemann
por: Kovács, Richard, et al.
Publicado: (2017)

Reliability of maximal mitochondrial oxidative phosphorylation in permeabilized fibers from the vastus lateralis employing high‐resolution respirometry
por: Cardinale, Daniele A., et al.
Publicado: (2018)

Control of actin polymerization in live and permeabilized fibroblasts
Publicado: (1991)

Regulation of apoptosis-associated lysosomal membrane permeabilization
por: Johansson, Ann-Charlotte, et al.
Publicado: (2010)

Vector-free intracellular delivery by reversible permeabilization
por: O’Dea, Shirley, et al.
Publicado: (2017)

Measurement of mitochondrial respiration in permeabilized fish gills
por: Dawson, Neal J., et al.
Publicado: (2020)

Mechanisms of Photosensitized Lipid Oxidation and Membrane Permeabilization
por: Bacellar, Isabel O. L., et al.
Publicado: (2019)

On the Mechanism of Membrane Permeabilization by Tamoxifen and 4-Hydroxytamoxifen
por: Ortiz, Julia, et al.
Publicado: (2023)

Effects of axon branching and asymmetry between the branches on transport, mean age, and age density distributions of mitochondria in neurons: A computational study
por: Kuznetsov, Ivan A., et al.
Publicado: (2022)

Rapid mitogen-induced aminopeptidase N surface expression in human T cells is dominated by mechanisms independent of de novo protein biosynthesis
por: Lendeckel, Uwe, et al.
Publicado: (1997)

The Complex Interplay between Mitochondria, ROS and Entire Cellular Metabolism
por: Kuznetsov, Andrey V., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_i8nflbmeonne3974d56ti7p8o2