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Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C

Effects of fatty acids on translocation of the γ- and ε-subspecies of protein kinase C (PKC) in living cells were investigated using their proteins fused with green fluorescent protein (GFP). γ-PKC–GFP and ε-PKC–GFP predominated in the cytoplasm, but only a small amount of γ-PKC–GFP was found in the...

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Autores principales: Shirai, Yasuhito, Kashiwagi, Kaori, Yagi, Keiko, Sakai, Norio, Saito, Naoaki
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132830/
https://www.ncbi.nlm.nih.gov/pubmed/9786959
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author Shirai, Yasuhito
Kashiwagi, Kaori
Yagi, Keiko
Sakai, Norio
Saito, Naoaki
author_facet Shirai, Yasuhito
Kashiwagi, Kaori
Yagi, Keiko
Sakai, Norio
Saito, Naoaki
author_sort Shirai, Yasuhito
collection PubMed
description Effects of fatty acids on translocation of the γ- and ε-subspecies of protein kinase C (PKC) in living cells were investigated using their proteins fused with green fluorescent protein (GFP). γ-PKC–GFP and ε-PKC–GFP predominated in the cytoplasm, but only a small amount of γ-PKC–GFP was found in the nucleus. Except at a high concentration of linoleic acid, all the fatty acids examined induced the translocation of γ-PKC–GFP from the cytoplasm to the plasma membrane within 30 s with a return to the cytoplasm in 3 min, but they had no effect on γ-PKC–GFP in the nucleus. Arachidonic and linoleic acids induced slow translocation of ε-PKC–GFP from the cytoplasm to the perinuclear region, whereas the other fatty acids (except for palmitic acid) induced rapid translocation to the plasma membrane. The target site of the slower translocation of ε-PKC–GFP by arachidonic acid was identified as the Golgi network. The critical concentration of fatty acid that induced translocation varied among the 11 fatty acids tested. In general, a higher concentration was required to induce the translocation of ε-PKC–GFP than that of γ-PKC–GFP, the exceptions being tridecanoic acid, linoleic acid, and arachidonic acid. Furthermore, arachidonic acid and the diacylglycerol analogue (DiC8) had synergistic effects on the translocation of γ-PKC–GFP. Simultaneous application of arachidonic acid (25 μM) and DiC8 (10 μM) elicited a slow, irreversible translocation of γ-PKC– GFP from the cytoplasm to the plasma membrane after rapid, reversible translocation, but a single application of arachidonic acid or DiC8 at the same concentration induced no translocation. These findings confirm the involvement of fatty acids in the translocation of γ- and ε-PKC, and they also indicate that each subspecies has a specific targeting mechanism that depends on the extracellular signals and that a combination of intracellular activators alters the target site of PKCs.
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spelling pubmed-21328302008-05-01 Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C Shirai, Yasuhito Kashiwagi, Kaori Yagi, Keiko Sakai, Norio Saito, Naoaki J Cell Biol Regular Articles Effects of fatty acids on translocation of the γ- and ε-subspecies of protein kinase C (PKC) in living cells were investigated using their proteins fused with green fluorescent protein (GFP). γ-PKC–GFP and ε-PKC–GFP predominated in the cytoplasm, but only a small amount of γ-PKC–GFP was found in the nucleus. Except at a high concentration of linoleic acid, all the fatty acids examined induced the translocation of γ-PKC–GFP from the cytoplasm to the plasma membrane within 30 s with a return to the cytoplasm in 3 min, but they had no effect on γ-PKC–GFP in the nucleus. Arachidonic and linoleic acids induced slow translocation of ε-PKC–GFP from the cytoplasm to the perinuclear region, whereas the other fatty acids (except for palmitic acid) induced rapid translocation to the plasma membrane. The target site of the slower translocation of ε-PKC–GFP by arachidonic acid was identified as the Golgi network. The critical concentration of fatty acid that induced translocation varied among the 11 fatty acids tested. In general, a higher concentration was required to induce the translocation of ε-PKC–GFP than that of γ-PKC–GFP, the exceptions being tridecanoic acid, linoleic acid, and arachidonic acid. Furthermore, arachidonic acid and the diacylglycerol analogue (DiC8) had synergistic effects on the translocation of γ-PKC–GFP. Simultaneous application of arachidonic acid (25 μM) and DiC8 (10 μM) elicited a slow, irreversible translocation of γ-PKC– GFP from the cytoplasm to the plasma membrane after rapid, reversible translocation, but a single application of arachidonic acid or DiC8 at the same concentration induced no translocation. These findings confirm the involvement of fatty acids in the translocation of γ- and ε-PKC, and they also indicate that each subspecies has a specific targeting mechanism that depends on the extracellular signals and that a combination of intracellular activators alters the target site of PKCs. The Rockefeller University Press 1998-10-19 /pmc/articles/PMC2132830/ /pubmed/9786959 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Regular Articles
Shirai, Yasuhito
Kashiwagi, Kaori
Yagi, Keiko
Sakai, Norio
Saito, Naoaki
Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C
title Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C
title_full Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C
title_fullStr Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C
title_full_unstemmed Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C
title_short Distinct Effects of Fatty Acids on Translocation of γ- and ε-Subspecies of Protein Kinase C
title_sort distinct effects of fatty acids on translocation of γ- and ε-subspecies of protein kinase c
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132830/
https://www.ncbi.nlm.nih.gov/pubmed/9786959
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