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CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation
CDO, a member of the Ig/fibronectin type III repeat subfamily of transmembrane proteins that includes the axon guidance receptor Robo, was identified by virtue of its down-regulation by the ras oncogene. We report here that one prominent site of cdo mRNA expression during murine embryogenesis is the...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132836/ https://www.ncbi.nlm.nih.gov/pubmed/9786951 |
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author | Kang, Jong-Sun Mulieri, Philip J. Miller, Cary Sassoon, David A. Krauss, Robert S. |
author_facet | Kang, Jong-Sun Mulieri, Philip J. Miller, Cary Sassoon, David A. Krauss, Robert S. |
author_sort | Kang, Jong-Sun |
collection | PubMed |
description | CDO, a member of the Ig/fibronectin type III repeat subfamily of transmembrane proteins that includes the axon guidance receptor Robo, was identified by virtue of its down-regulation by the ras oncogene. We report here that one prominent site of cdo mRNA expression during murine embryogenesis is the early myogenic compartment (newly formed somites, dermomyotome and myotome). CDO is expressed in proliferating and differentiating C2C12 myoblasts and in myoblast lines derived by treating 10T1/2 fibroblasts with 5-azacytidine, but not in parental 10T1/2 cells. Overexpression of CDO in C2C12 cells accelerates differentiation, while expression of secreted soluble extracellular regions of CDO inhibits this process. Oncogenic Ras is known to block differentiation of C2C12 cells via downregulation of MyoD. Reexpression of CDO in C2C12/Ras cells induces MyoD; conversely, MyoD induces CDO. Reexpression of either CDO or MyoD rescues differentiation of C2C12/Ras cells without altering anchorage-independent growth or morphological transformation. CDO and MyoD are therefore involved in a positive feedback loop that is central to the inverse relationship between cell differentiation and transformation. It is proposed that CDO mediates, at least in part, the effects of cell–cell interactions between muscle precursors that are critical in myogenesis. |
format | Text |
id | pubmed-2132836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21328362008-05-01 CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation Kang, Jong-Sun Mulieri, Philip J. Miller, Cary Sassoon, David A. Krauss, Robert S. J Cell Biol Regular Articles CDO, a member of the Ig/fibronectin type III repeat subfamily of transmembrane proteins that includes the axon guidance receptor Robo, was identified by virtue of its down-regulation by the ras oncogene. We report here that one prominent site of cdo mRNA expression during murine embryogenesis is the early myogenic compartment (newly formed somites, dermomyotome and myotome). CDO is expressed in proliferating and differentiating C2C12 myoblasts and in myoblast lines derived by treating 10T1/2 fibroblasts with 5-azacytidine, but not in parental 10T1/2 cells. Overexpression of CDO in C2C12 cells accelerates differentiation, while expression of secreted soluble extracellular regions of CDO inhibits this process. Oncogenic Ras is known to block differentiation of C2C12 cells via downregulation of MyoD. Reexpression of CDO in C2C12/Ras cells induces MyoD; conversely, MyoD induces CDO. Reexpression of either CDO or MyoD rescues differentiation of C2C12/Ras cells without altering anchorage-independent growth or morphological transformation. CDO and MyoD are therefore involved in a positive feedback loop that is central to the inverse relationship between cell differentiation and transformation. It is proposed that CDO mediates, at least in part, the effects of cell–cell interactions between muscle precursors that are critical in myogenesis. The Rockefeller University Press 1998-10-19 /pmc/articles/PMC2132836/ /pubmed/9786951 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Regular Articles Kang, Jong-Sun Mulieri, Philip J. Miller, Cary Sassoon, David A. Krauss, Robert S. CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation |
title | CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation |
title_full | CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation |
title_fullStr | CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation |
title_full_unstemmed | CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation |
title_short | CDO, A Robo-related Cell Surface Protein that Mediates Myogenic Differentiation |
title_sort | cdo, a robo-related cell surface protein that mediates myogenic differentiation |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132836/ https://www.ncbi.nlm.nih.gov/pubmed/9786951 |
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