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Functional Differences between Keratins of Stratified and Simple Epithelia

Dividing populations of stratified and simple epithelial tissues express keratins 5 and 14, and keratins 8 and 18, respectively. It has been suggested that these keratins form a mechanical framework important to cellular integrity, since their absence gives rise to a blistering skin disorder in neon...

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Autores principales: Hutton, Elizabeth, Paladini, Rudolph D., Yu, Qian-Chun, Yen, Mei, Coulombe, Pierre A., Fuchs, Elaine
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132837/
https://www.ncbi.nlm.nih.gov/pubmed/9786957
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author Hutton, Elizabeth
Paladini, Rudolph D.
Yu, Qian-Chun
Yen, Mei
Coulombe, Pierre A.
Fuchs, Elaine
author_facet Hutton, Elizabeth
Paladini, Rudolph D.
Yu, Qian-Chun
Yen, Mei
Coulombe, Pierre A.
Fuchs, Elaine
author_sort Hutton, Elizabeth
collection PubMed
description Dividing populations of stratified and simple epithelial tissues express keratins 5 and 14, and keratins 8 and 18, respectively. It has been suggested that these keratins form a mechanical framework important to cellular integrity, since their absence gives rise to a blistering skin disorder in neonatal epidermis, and hemorrhaging within the embryonic liver. An unresolved fundamental issue is whether different keratins perform unique functions in epithelia. We now address this question using transgenic technology to express a K16-14 hybrid epidermal keratin transgene and a K18 simple epithelial keratin transgene in the epidermis of mice null for K14. Under conditions where the hybrid epidermal keratin restored a wild-type phenotype to newborn epidermis, K18 partially but not fully rescued. The explanation does not appear to reside in an inability of K18 to form 10-nm filaments with K5, which it does in vitro and in vivo. Rather, it appears that the keratin network formed between K5 and K18 is deficient in withstanding mechanical stress, leading to perturbations in the keratin network in regions of the skin that are subjected either to natural or to mechanically induced trauma. Taken together, these findings suggest that the loss of a type I epidermal keratin cannot be fully compensated by its counterpart of simple epithelial cells, and that in vivo, all keratins are not equivalent.
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spelling pubmed-21328372008-05-01 Functional Differences between Keratins of Stratified and Simple Epithelia Hutton, Elizabeth Paladini, Rudolph D. Yu, Qian-Chun Yen, Mei Coulombe, Pierre A. Fuchs, Elaine J Cell Biol Regular Articles Dividing populations of stratified and simple epithelial tissues express keratins 5 and 14, and keratins 8 and 18, respectively. It has been suggested that these keratins form a mechanical framework important to cellular integrity, since their absence gives rise to a blistering skin disorder in neonatal epidermis, and hemorrhaging within the embryonic liver. An unresolved fundamental issue is whether different keratins perform unique functions in epithelia. We now address this question using transgenic technology to express a K16-14 hybrid epidermal keratin transgene and a K18 simple epithelial keratin transgene in the epidermis of mice null for K14. Under conditions where the hybrid epidermal keratin restored a wild-type phenotype to newborn epidermis, K18 partially but not fully rescued. The explanation does not appear to reside in an inability of K18 to form 10-nm filaments with K5, which it does in vitro and in vivo. Rather, it appears that the keratin network formed between K5 and K18 is deficient in withstanding mechanical stress, leading to perturbations in the keratin network in regions of the skin that are subjected either to natural or to mechanically induced trauma. Taken together, these findings suggest that the loss of a type I epidermal keratin cannot be fully compensated by its counterpart of simple epithelial cells, and that in vivo, all keratins are not equivalent. The Rockefeller University Press 1998-10-19 /pmc/articles/PMC2132837/ /pubmed/9786957 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Regular Articles
Hutton, Elizabeth
Paladini, Rudolph D.
Yu, Qian-Chun
Yen, Mei
Coulombe, Pierre A.
Fuchs, Elaine
Functional Differences between Keratins of Stratified and Simple Epithelia
title Functional Differences between Keratins of Stratified and Simple Epithelia
title_full Functional Differences between Keratins of Stratified and Simple Epithelia
title_fullStr Functional Differences between Keratins of Stratified and Simple Epithelia
title_full_unstemmed Functional Differences between Keratins of Stratified and Simple Epithelia
title_short Functional Differences between Keratins of Stratified and Simple Epithelia
title_sort functional differences between keratins of stratified and simple epithelia
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132837/
https://www.ncbi.nlm.nih.gov/pubmed/9786957
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