pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts

We have isolated a cDNA, Eg7, corresponding to a Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylated in early embryos. This maternal mRNA encodes a protein, pEg7, whose expression is strongly increased during oocyte maturation. The tissue and cell expression pattern of...

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Autores principales: Cubizolles, Fabien, Legagneux, Vincent, Le Guellec, René, Chartrain, Isabelle, Uzbekov, Rustem, Ford, Chris, Le Guellec, Katherine
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132990/
https://www.ncbi.nlm.nih.gov/pubmed/9852142
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author Cubizolles, Fabien
Legagneux, Vincent
Le Guellec, René
Chartrain, Isabelle
Uzbekov, Rustem
Ford, Chris
Le Guellec, Katherine
author_facet Cubizolles, Fabien
Legagneux, Vincent
Le Guellec, René
Chartrain, Isabelle
Uzbekov, Rustem
Ford, Chris
Le Guellec, Katherine
author_sort Cubizolles, Fabien
collection PubMed
description We have isolated a cDNA, Eg7, corresponding to a Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylated in early embryos. This maternal mRNA encodes a protein, pEg7, whose expression is strongly increased during oocyte maturation. The tissue and cell expression pattern of pEg7 indicates that this protein is only readily detected in cultured cells and germ cells. Immunolocalization in Xenopus cultured cells indicates that pEg7 concentrates onto chromosomes during mitosis. A similar localization of pEg7 is observed when sperm chromatin is allowed to form mitotic chromosomes in cytostatic factor-arrested egg extracts. Incubating these extracts with antibodies directed against two distinct parts of pEg7 provokes a strong inhibition of the condensation and resolution of mitotic chromosomes. Biochemical experiments show that pEg7 associates with Xenopus chromosome-associated polypeptides C and E, two components of the 13S condensin.
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spelling pubmed-21329902008-05-01 pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts Cubizolles, Fabien Legagneux, Vincent Le Guellec, René Chartrain, Isabelle Uzbekov, Rustem Ford, Chris Le Guellec, Katherine J Cell Biol Article We have isolated a cDNA, Eg7, corresponding to a Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylated in early embryos. This maternal mRNA encodes a protein, pEg7, whose expression is strongly increased during oocyte maturation. The tissue and cell expression pattern of pEg7 indicates that this protein is only readily detected in cultured cells and germ cells. Immunolocalization in Xenopus cultured cells indicates that pEg7 concentrates onto chromosomes during mitosis. A similar localization of pEg7 is observed when sperm chromatin is allowed to form mitotic chromosomes in cytostatic factor-arrested egg extracts. Incubating these extracts with antibodies directed against two distinct parts of pEg7 provokes a strong inhibition of the condensation and resolution of mitotic chromosomes. Biochemical experiments show that pEg7 associates with Xenopus chromosome-associated polypeptides C and E, two components of the 13S condensin. The Rockefeller University Press 1998-12-14 /pmc/articles/PMC2132990/ /pubmed/9852142 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Cubizolles, Fabien
Legagneux, Vincent
Le Guellec, René
Chartrain, Isabelle
Uzbekov, Rustem
Ford, Chris
Le Guellec, Katherine
pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts
title pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts
title_full pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts
title_fullStr pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts
title_full_unstemmed pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts
title_short pEg7, a New Xenopus Protein Required for Mitotic Chromosome Condensation in Egg Extracts
title_sort peg7, a new xenopus protein required for mitotic chromosome condensation in egg extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132990/
https://www.ncbi.nlm.nih.gov/pubmed/9852142
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