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Caspase-independent Cell Killing by Fas-associated Protein with Death Domain

The binding of Fas ligand to Fas recruits caspase 8 to Fas via an adaptor, FADD/MORT1, and activates a caspase cascade leading to apoptosis. Here, we describe a human Jurkat-derived cell line (JB-6) that is deficient in caspase 8. This cell line was resistant to the apoptosis triggered by Fas engage...

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Detalles Bibliográficos
Autores principales: Kawahara, Atsuo, Ohsawa, Yoshiyuki, Matsumura, Hirotaka, Uchiyama, Yasuo, Nagata, Shigekazu
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133085/
https://www.ncbi.nlm.nih.gov/pubmed/9832562
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author Kawahara, Atsuo
Ohsawa, Yoshiyuki
Matsumura, Hirotaka
Uchiyama, Yasuo
Nagata, Shigekazu
author_facet Kawahara, Atsuo
Ohsawa, Yoshiyuki
Matsumura, Hirotaka
Uchiyama, Yasuo
Nagata, Shigekazu
author_sort Kawahara, Atsuo
collection PubMed
description The binding of Fas ligand to Fas recruits caspase 8 to Fas via an adaptor, FADD/MORT1, and activates a caspase cascade leading to apoptosis. Here, we describe a human Jurkat-derived cell line (JB-6) that is deficient in caspase 8. This cell line was resistant to the apoptosis triggered by Fas engagement. However, the multimerization of Fas-associated protein with death domain, through the use of a dimerizing system, killed the JB-6 cells. This killing process was not accompanied by the activation of caspases or DNA fragmentation. The dying cells showed neither condensation nor fragmentation of cells and nuclei, but the cells and nuclei swelled in a manner similar to that seen in necrosis. These results suggested that Fas-associated protein with death domain can kill the cells via two pathways, one mediated by caspases and another that does not involve them.
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spelling pubmed-21330852008-05-01 Caspase-independent Cell Killing by Fas-associated Protein with Death Domain Kawahara, Atsuo Ohsawa, Yoshiyuki Matsumura, Hirotaka Uchiyama, Yasuo Nagata, Shigekazu J Cell Biol Article The binding of Fas ligand to Fas recruits caspase 8 to Fas via an adaptor, FADD/MORT1, and activates a caspase cascade leading to apoptosis. Here, we describe a human Jurkat-derived cell line (JB-6) that is deficient in caspase 8. This cell line was resistant to the apoptosis triggered by Fas engagement. However, the multimerization of Fas-associated protein with death domain, through the use of a dimerizing system, killed the JB-6 cells. This killing process was not accompanied by the activation of caspases or DNA fragmentation. The dying cells showed neither condensation nor fragmentation of cells and nuclei, but the cells and nuclei swelled in a manner similar to that seen in necrosis. These results suggested that Fas-associated protein with death domain can kill the cells via two pathways, one mediated by caspases and another that does not involve them. The Rockefeller University Press 1998-11-30 /pmc/articles/PMC2133085/ /pubmed/9832562 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kawahara, Atsuo
Ohsawa, Yoshiyuki
Matsumura, Hirotaka
Uchiyama, Yasuo
Nagata, Shigekazu
Caspase-independent Cell Killing by Fas-associated Protein with Death Domain
title Caspase-independent Cell Killing by Fas-associated Protein with Death Domain
title_full Caspase-independent Cell Killing by Fas-associated Protein with Death Domain
title_fullStr Caspase-independent Cell Killing by Fas-associated Protein with Death Domain
title_full_unstemmed Caspase-independent Cell Killing by Fas-associated Protein with Death Domain
title_short Caspase-independent Cell Killing by Fas-associated Protein with Death Domain
title_sort caspase-independent cell killing by fas-associated protein with death domain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133085/
https://www.ncbi.nlm.nih.gov/pubmed/9832562
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