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Caspase-independent Cell Killing by Fas-associated Protein with Death Domain
The binding of Fas ligand to Fas recruits caspase 8 to Fas via an adaptor, FADD/MORT1, and activates a caspase cascade leading to apoptosis. Here, we describe a human Jurkat-derived cell line (JB-6) that is deficient in caspase 8. This cell line was resistant to the apoptosis triggered by Fas engage...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133085/ https://www.ncbi.nlm.nih.gov/pubmed/9832562 |
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author | Kawahara, Atsuo Ohsawa, Yoshiyuki Matsumura, Hirotaka Uchiyama, Yasuo Nagata, Shigekazu |
author_facet | Kawahara, Atsuo Ohsawa, Yoshiyuki Matsumura, Hirotaka Uchiyama, Yasuo Nagata, Shigekazu |
author_sort | Kawahara, Atsuo |
collection | PubMed |
description | The binding of Fas ligand to Fas recruits caspase 8 to Fas via an adaptor, FADD/MORT1, and activates a caspase cascade leading to apoptosis. Here, we describe a human Jurkat-derived cell line (JB-6) that is deficient in caspase 8. This cell line was resistant to the apoptosis triggered by Fas engagement. However, the multimerization of Fas-associated protein with death domain, through the use of a dimerizing system, killed the JB-6 cells. This killing process was not accompanied by the activation of caspases or DNA fragmentation. The dying cells showed neither condensation nor fragmentation of cells and nuclei, but the cells and nuclei swelled in a manner similar to that seen in necrosis. These results suggested that Fas-associated protein with death domain can kill the cells via two pathways, one mediated by caspases and another that does not involve them. |
format | Text |
id | pubmed-2133085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21330852008-05-01 Caspase-independent Cell Killing by Fas-associated Protein with Death Domain Kawahara, Atsuo Ohsawa, Yoshiyuki Matsumura, Hirotaka Uchiyama, Yasuo Nagata, Shigekazu J Cell Biol Article The binding of Fas ligand to Fas recruits caspase 8 to Fas via an adaptor, FADD/MORT1, and activates a caspase cascade leading to apoptosis. Here, we describe a human Jurkat-derived cell line (JB-6) that is deficient in caspase 8. This cell line was resistant to the apoptosis triggered by Fas engagement. However, the multimerization of Fas-associated protein with death domain, through the use of a dimerizing system, killed the JB-6 cells. This killing process was not accompanied by the activation of caspases or DNA fragmentation. The dying cells showed neither condensation nor fragmentation of cells and nuclei, but the cells and nuclei swelled in a manner similar to that seen in necrosis. These results suggested that Fas-associated protein with death domain can kill the cells via two pathways, one mediated by caspases and another that does not involve them. The Rockefeller University Press 1998-11-30 /pmc/articles/PMC2133085/ /pubmed/9832562 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kawahara, Atsuo Ohsawa, Yoshiyuki Matsumura, Hirotaka Uchiyama, Yasuo Nagata, Shigekazu Caspase-independent Cell Killing by Fas-associated Protein with Death Domain |
title | Caspase-independent Cell Killing by Fas-associated Protein with Death Domain |
title_full | Caspase-independent Cell Killing by Fas-associated Protein with Death Domain |
title_fullStr | Caspase-independent Cell Killing by Fas-associated Protein with Death Domain |
title_full_unstemmed | Caspase-independent Cell Killing by Fas-associated Protein with Death Domain |
title_short | Caspase-independent Cell Killing by Fas-associated Protein with Death Domain |
title_sort | caspase-independent cell killing by fas-associated protein with death domain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133085/ https://www.ncbi.nlm.nih.gov/pubmed/9832562 |
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