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THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS
1. The enzyme which decomposes the capsular polysaccharide of Type III Pneumococcus is associated with a protein which under optimal conditions of filtration passes through membranes with an average pore size of 10.6 mµ but is held back by pores having a diameter of 8.2 mµ. 2. When enzyme solutions...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1935
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133266/ https://www.ncbi.nlm.nih.gov/pubmed/19870413 |
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author | Dubos, René Bauer, Johannes H. |
author_facet | Dubos, René Bauer, Johannes H. |
author_sort | Dubos, René |
collection | PubMed |
description | 1. The enzyme which decomposes the capsular polysaccharide of Type III Pneumococcus is associated with a protein which under optimal conditions of filtration passes through membranes with an average pore size of 10.6 mµ but is held back by pores having a diameter of 8.2 mµ. 2. When enzyme solutions are filtered to dryness through membranes of such porosity as to hold back the active principle, and when proper precautions are taken to prevent or minimize adsorption, the enzyme can be completely recovered in solution by immersing the membrane in distilled water or physiological salt solution. 3. These results are discussed with reference to the dimensions of the enzyme particle, and to the purification obtained in the course of ultrafiltration. 4. A practical method is described for the concentration and purification of the crude enzyme preparation by the use of graded collodion membranes. |
format | Text |
id | pubmed-2133266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1935 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21332662008-04-18 THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS Dubos, René Bauer, Johannes H. J Exp Med Article 1. The enzyme which decomposes the capsular polysaccharide of Type III Pneumococcus is associated with a protein which under optimal conditions of filtration passes through membranes with an average pore size of 10.6 mµ but is held back by pores having a diameter of 8.2 mµ. 2. When enzyme solutions are filtered to dryness through membranes of such porosity as to hold back the active principle, and when proper precautions are taken to prevent or minimize adsorption, the enzyme can be completely recovered in solution by immersing the membrane in distilled water or physiological salt solution. 3. These results are discussed with reference to the dimensions of the enzyme particle, and to the purification obtained in the course of ultrafiltration. 4. A practical method is described for the concentration and purification of the crude enzyme preparation by the use of graded collodion membranes. The Rockefeller University Press 1935-07-31 /pmc/articles/PMC2133266/ /pubmed/19870413 Text en Copyright © Copyright, 1935, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Dubos, René Bauer, Johannes H. THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS |
title | THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS |
title_full | THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS |
title_fullStr | THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS |
title_full_unstemmed | THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS |
title_short | THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS |
title_sort | use of graded collodion membranes for the concentration of a bacterial enzyme capable of decomposing the capsular polysaccharide of type iii pneumococcus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133266/ https://www.ncbi.nlm.nih.gov/pubmed/19870413 |
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