THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS

1. Fragments of breast muscle from a 12 day old chick embryo have been kept alive in single flasks for an entire year without being transferred. The nutrient materials were supplied by frequent applications of adult fowl serum diluted with Tyrode solution. 2. When fragments of fixed tissues are cult...

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Detalles Bibliográficos
Autor principal: Parker, Raymond C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1936
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133417/
https://www.ncbi.nlm.nih.gov/pubmed/19870516
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author Parker, Raymond C.
author_facet Parker, Raymond C.
author_sort Parker, Raymond C.
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description 1. Fragments of breast muscle from a 12 day old chick embryo have been kept alive in single flasks for an entire year without being transferred. The nutrient materials were supplied by frequent applications of adult fowl serum diluted with Tyrode solution. 2. When fragments of fixed tissues are cultivated in serum, cell multiplication and cell death are both reduced to an extremely low level. 3. The presence of a plasma coagulum is not essential to the continued survival and further development of tissues cultivated inserum. 4. The fibrinogen, prothrombin, and fibrin of coagulated plasma are not essential to the development of connective tissue fibers in vitro.
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spelling pubmed-21334172008-04-18 THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS Parker, Raymond C. J Exp Med Article 1. Fragments of breast muscle from a 12 day old chick embryo have been kept alive in single flasks for an entire year without being transferred. The nutrient materials were supplied by frequent applications of adult fowl serum diluted with Tyrode solution. 2. When fragments of fixed tissues are cultivated in serum, cell multiplication and cell death are both reduced to an extremely low level. 3. The presence of a plasma coagulum is not essential to the continued survival and further development of tissues cultivated inserum. 4. The fibrinogen, prothrombin, and fibrin of coagulated plasma are not essential to the development of connective tissue fibers in vitro. The Rockefeller University Press 1936-06-30 /pmc/articles/PMC2133417/ /pubmed/19870516 Text en Copyright © Copyright, 1936, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Parker, Raymond C.
THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS
title THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS
title_full THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS
title_fullStr THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS
title_full_unstemmed THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS
title_short THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS
title_sort cultivation of tissues for prolonged periods in single flasks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133417/
https://www.ncbi.nlm.nih.gov/pubmed/19870516
work_keys_str_mv AT parkerraymondc thecultivationoftissuesforprolongedperiodsinsingleflasks
AT parkerraymondc cultivationoftissuesforprolongedperiodsinsingleflasks

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