CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION

A quantitative biological assay of the products obtained from the dialysis of B. typhosus, meningococcus, and B. coli culture filtrates has been undertaken. It was found that the active principles of the phenomenon of local skin reactivity to bacterial filtrates were retained by cellophane membranes...

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Detalles Bibliográficos
Autores principales: Shwartzman, Gregory, Morell, Sam, Sobotka, Harry
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1937
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133496/
https://www.ncbi.nlm.nih.gov/pubmed/19870604
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author Shwartzman, Gregory
Morell, Sam
Sobotka, Harry
author_facet Shwartzman, Gregory
Morell, Sam
Sobotka, Harry
author_sort Shwartzman, Gregory
collection PubMed
description A quantitative biological assay of the products obtained from the dialysis of B. typhosus, meningococcus, and B. coli culture filtrates has been undertaken. It was found that the active principles of the phenomenon of local skin reactivity to bacterial filtrates were retained by cellophane membranes. An appreciable purification was thus effected, amounting, on the average, to about a threefold increase in reacting potency per milligram of dry weight and of nitrogen. Attempts to purify bacterial filtrates by the fractional precipitation of their concentrates with dilute alkali, acid, and alcohol were unsuccessful.
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spelling pubmed-21334962008-04-18 CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION Shwartzman, Gregory Morell, Sam Sobotka, Harry J Exp Med Article A quantitative biological assay of the products obtained from the dialysis of B. typhosus, meningococcus, and B. coli culture filtrates has been undertaken. It was found that the active principles of the phenomenon of local skin reactivity to bacterial filtrates were retained by cellophane membranes. An appreciable purification was thus effected, amounting, on the average, to about a threefold increase in reacting potency per milligram of dry weight and of nitrogen. Attempts to purify bacterial filtrates by the fractional precipitation of their concentrates with dilute alkali, acid, and alcohol were unsuccessful. The Rockefeller University Press 1937-02-28 /pmc/articles/PMC2133496/ /pubmed/19870604 Text en Copyright © Copyright, 1937, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Shwartzman, Gregory
Morell, Sam
Sobotka, Harry
CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION
title CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION
title_full CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION
title_fullStr CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION
title_full_unstemmed CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION
title_short CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES : I. PURIFICATION BY DIALYSIS, AND ATTEMPTS AT FRACTIONAL PRECIPITATION
title_sort chemical investigations on the active principles of the phenomenon of local skin reactivity to bacterial filtrates : i. purification by dialysis, and attempts at fractional precipitation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133496/
https://www.ncbi.nlm.nih.gov/pubmed/19870604
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AT morellsam chemicalinvestigationsontheactiveprinciplesofthephenomenonoflocalskinreactivitytobacterialfiltratesipurificationbydialysisandattemptsatfractionalprecipitation
AT sobotkaharry chemicalinvestigationsontheactiveprinciplesofthephenomenonoflocalskinreactivitytobacterialfiltratesipurificationbydialysisandattemptsatfractionalprecipitation

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