QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI

1. The salt dissociation and barium hydroxide-barium chloride methods are extended to the preparation of highly purified antibody solutions from specific precipitates derived from Type III and Type VIII antipneumococcus horse sera and a low grade polyvalent bovine serum. Analytically pure precipitin...

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Autores principales: Heidelberger, Michael, Kabat, Elvin A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1938
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133561/
https://www.ncbi.nlm.nih.gov/pubmed/19870714
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author Heidelberger, Michael
Kabat, Elvin A.
author_facet Heidelberger, Michael
Kabat, Elvin A.
author_sort Heidelberger, Michael
collection PubMed
description 1. The salt dissociation and barium hydroxide-barium chloride methods are extended to the preparation of highly purified antibody solutions from specific precipitates derived from Type III and Type VIII antipneumococcus horse sera and a low grade polyvalent bovine serum. Analytically pure precipitin (agglutinin) was obtained from the last, and Types I, II, and III antibodies were separated. 2. Difficulties connected with the application of the methods to Type I antipneumococcus rabbit sera are described, as is also the purification of antibody from low grade pig and sheep sera. 3. The dissociation of antibody by both methods from Type I pneumococci agglutinated in antisera produced in the horse, rabbit, pig, and sheep, is described and its advantages discussed. 4. Certain theoretical aspects of the work are also discussed.
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spelling pubmed-21335612008-04-18 QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI Heidelberger, Michael Kabat, Elvin A. J Exp Med Article 1. The salt dissociation and barium hydroxide-barium chloride methods are extended to the preparation of highly purified antibody solutions from specific precipitates derived from Type III and Type VIII antipneumococcus horse sera and a low grade polyvalent bovine serum. Analytically pure precipitin (agglutinin) was obtained from the last, and Types I, II, and III antibodies were separated. 2. Difficulties connected with the application of the methods to Type I antipneumococcus rabbit sera are described, as is also the purification of antibody from low grade pig and sheep sera. 3. The dissociation of antibody by both methods from Type I pneumococci agglutinated in antisera produced in the horse, rabbit, pig, and sheep, is described and its advantages discussed. 4. Certain theoretical aspects of the work are also discussed. The Rockefeller University Press 1938-01-31 /pmc/articles/PMC2133561/ /pubmed/19870714 Text en Copyright © Copyright, 1938, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Heidelberger, Michael
Kabat, Elvin A.
QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI
title QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI
title_full QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI
title_fullStr QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI
title_full_unstemmed QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI
title_short QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : II. THE DISSOCIATION OF ANTIBODY FROM PNEUMOCOCCUS SPECIFIC PRECIPITATES AND SPECIFICALLY AGGLUTINATED PNEUMOCOCCI
title_sort quantitative studies on antibody purification : ii. the dissociation of antibody from pneumococcus specific precipitates and specifically agglutinated pneumococci
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133561/
https://www.ncbi.nlm.nih.gov/pubmed/19870714
work_keys_str_mv AT heidelbergermichael quantitativestudiesonantibodypurificationiithedissociationofantibodyfrompneumococcusspecificprecipitatesandspecificallyagglutinatedpneumococci
AT kabatelvina quantitativestudiesonantibodypurificationiithedissociationofantibodyfrompneumococcusspecificprecipitatesandspecificallyagglutinatedpneumococci

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