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THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI
1. Mice immunized with heat-killed cells of virulent pneumococci (Type I) which have been treated with active preparations of the bacteriolytic enzyme, develop a certain degree of type specific resistance to subsequent infection. This active immunity, however, appears to be due to the small amount o...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1937
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133591/ https://www.ncbi.nlm.nih.gov/pubmed/19870645 |
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author | Dubos, René J. |
author_facet | Dubos, René J. |
author_sort | Dubos, René J. |
collection | PubMed |
description | 1. Mice immunized with heat-killed cells of virulent pneumococci (Type I) which have been treated with active preparations of the bacteriolytic enzyme, develop a certain degree of type specific resistance to subsequent infection. This active immunity, however, appears to be due to the small amount of free acetyl polysaccharide present in the suspension of digested bacteria, and is always of a less pronounced degree than that obtained with intact heat-killed cells. 2. Virulent pneumococci killed by heat or iodine when subjected to the action of active preparations of the bacteriolytic enzyme lose the antigenic property of stimulating in rabbits the formation of precipitating antibodies for the type specific polysaccharide. 3. The enzyme prepared from S or R pneumococci, irrespective of type derivation, is equally effective against the capsular polysaccharide antigen of any specific type of this bacterial species. 4. The inactivation of the capsular polysaccharide antigen is observed when the cells are merely rendered Gram-negative, without being caused to undergo actual disintegration or lysis. 5. These observations emphasize the importance of minimizing the chances of alterations due to the action of cellular enzymes in the course of preparation of the cell suspension to be used as immunizing agents. |
format | Text |
id | pubmed-2133591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1937 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21335912008-04-18 THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI Dubos, René J. J Exp Med Article 1. Mice immunized with heat-killed cells of virulent pneumococci (Type I) which have been treated with active preparations of the bacteriolytic enzyme, develop a certain degree of type specific resistance to subsequent infection. This active immunity, however, appears to be due to the small amount of free acetyl polysaccharide present in the suspension of digested bacteria, and is always of a less pronounced degree than that obtained with intact heat-killed cells. 2. Virulent pneumococci killed by heat or iodine when subjected to the action of active preparations of the bacteriolytic enzyme lose the antigenic property of stimulating in rabbits the formation of precipitating antibodies for the type specific polysaccharide. 3. The enzyme prepared from S or R pneumococci, irrespective of type derivation, is equally effective against the capsular polysaccharide antigen of any specific type of this bacterial species. 4. The inactivation of the capsular polysaccharide antigen is observed when the cells are merely rendered Gram-negative, without being caused to undergo actual disintegration or lysis. 5. These observations emphasize the importance of minimizing the chances of alterations due to the action of cellular enzymes in the course of preparation of the cell suspension to be used as immunizing agents. The Rockefeller University Press 1937-06-30 /pmc/articles/PMC2133591/ /pubmed/19870645 Text en Copyright © Copyright, 1937, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Dubos, René J. THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI |
title | THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI |
title_full | THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI |
title_fullStr | THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI |
title_full_unstemmed | THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI |
title_short | THE EFFECT OF THE BACTERIOLYTIC ENZYME OF PNEUMOCOCCUS UPON THE ANTIGENICITY OF ENCAPSULATED PNEUMOCOCCI |
title_sort | effect of the bacteriolytic enzyme of pneumococcus upon the antigenicity of encapsulated pneumococci |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133591/ https://www.ncbi.nlm.nih.gov/pubmed/19870645 |
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