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A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS

1. Quantitative data for both homologous and heterologous precipitin reactions of human, hog, beef, and sheep thyroglobulins show that these reactions have the same mechanism as other instances of the precipitin reaction and may be expressed quantitatively by the same equations derived from the law...

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Detalles Bibliográficos
Autores principales: Stokinger, Herbert E., Heidelberger, Michael
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1937
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133596/
https://www.ncbi.nlm.nih.gov/pubmed/19870659
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author Stokinger, Herbert E.
Heidelberger, Michael
author_facet Stokinger, Herbert E.
Heidelberger, Michael
author_sort Stokinger, Herbert E.
collection PubMed
description 1. Quantitative data for both homologous and heterologous precipitin reactions of human, hog, beef, and sheep thyroglobulins show that these reactions have the same mechanism as other instances of the precipitin reaction and may be expressed quantitatively by the same equations derived from the law of mass action. 2. It is shown that all of the added antigen is precipitated in the region of antibody excess and in the equivalence zone, so that in these portions of the reaction range the composition of the specific precipitate may be calculated from the nitrogen precipitated and the amount of antigen nitrogen added. 3. The thyroglobulin-antibody reaction is characterized by low antibody N to antigen N ratios, as would be expected with an antigen of high molecular weight. Molecular ratios varying from 60:1 to 1:1 were calculated for the extremes of the reaction range, indicating a very large number of immunologically reactive groupings on the thyroglobulin molecule. 4. Failure of thyroxine or diiodotyrosine to inhibit specific precipitation was confirmed, but it is shown that this need not mean that these substances do not occur in thyroglobulin, as has been claimed.
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spelling pubmed-21335962008-04-18 A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS Stokinger, Herbert E. Heidelberger, Michael J Exp Med Article 1. Quantitative data for both homologous and heterologous precipitin reactions of human, hog, beef, and sheep thyroglobulins show that these reactions have the same mechanism as other instances of the precipitin reaction and may be expressed quantitatively by the same equations derived from the law of mass action. 2. It is shown that all of the added antigen is precipitated in the region of antibody excess and in the equivalence zone, so that in these portions of the reaction range the composition of the specific precipitate may be calculated from the nitrogen precipitated and the amount of antigen nitrogen added. 3. The thyroglobulin-antibody reaction is characterized by low antibody N to antigen N ratios, as would be expected with an antigen of high molecular weight. Molecular ratios varying from 60:1 to 1:1 were calculated for the extremes of the reaction range, indicating a very large number of immunologically reactive groupings on the thyroglobulin molecule. 4. Failure of thyroxine or diiodotyrosine to inhibit specific precipitation was confirmed, but it is shown that this need not mean that these substances do not occur in thyroglobulin, as has been claimed. The Rockefeller University Press 1937-07-31 /pmc/articles/PMC2133596/ /pubmed/19870659 Text en Copyright © Copyright, 1937, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Stokinger, Herbert E.
Heidelberger, Michael
A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS
title A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS
title_full A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS
title_fullStr A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS
title_full_unstemmed A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS
title_short A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS
title_sort quantitative theory of the precipitin reaction : vi. the reaction between mammalian thyroglobulins and antibodies to homologous and heterologous preparations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133596/
https://www.ncbi.nlm.nih.gov/pubmed/19870659
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