A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE

A method is presented for determining the relative degree of sedimentation of proteins and other small particles, either in pure form or in a mixture, utilizing the concentration centrifuge of Bauer and Pickels. Chemical analysis or biological assay of material obtained at different fluid levels by...

Descripción completa

Detalles Bibliográficos
Autores principales: Hughes, Thomas P., Pickels, Edward G., Horsfall, Frank L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1938
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133646/
https://www.ncbi.nlm.nih.gov/pubmed/19870767
_version_ 1782142718237999104
author Hughes, Thomas P.
Pickels, Edward G.
Horsfall, Frank L.
author_facet Hughes, Thomas P.
Pickels, Edward G.
Horsfall, Frank L.
author_sort Hughes, Thomas P.
collection PubMed
description A method is presented for determining the relative degree of sedimentation of proteins and other small particles, either in pure form or in a mixture, utilizing the concentration centrifuge of Bauer and Pickels. Chemical analysis or biological assay of material obtained at different fluid levels by means of a special sampling device is used to measure the degree of sedimentation. The results are reproducible within 10 per cent and, under the conditions of the experiments cited, are characteristic for the proteins thus far investigated.
format Text
id pubmed-2133646
institution National Center for Biotechnology Information
language English
publishDate 1938
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21336462008-04-18 A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE Hughes, Thomas P. Pickels, Edward G. Horsfall, Frank L. J Exp Med Article A method is presented for determining the relative degree of sedimentation of proteins and other small particles, either in pure form or in a mixture, utilizing the concentration centrifuge of Bauer and Pickels. Chemical analysis or biological assay of material obtained at different fluid levels by means of a special sampling device is used to measure the degree of sedimentation. The results are reproducible within 10 per cent and, under the conditions of the experiments cited, are characteristic for the proteins thus far investigated. The Rockefeller University Press 1938-05-31 /pmc/articles/PMC2133646/ /pubmed/19870767 Text en Copyright © Copyright, 1938, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hughes, Thomas P.
Pickels, Edward G.
Horsfall, Frank L.
A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE
title A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE
title_full A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE
title_fullStr A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE
title_full_unstemmed A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE
title_short A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE
title_sort method for determining the differential sedimentation of proteins in the high speed concentration centrifuge
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133646/
https://www.ncbi.nlm.nih.gov/pubmed/19870767
work_keys_str_mv AT hughesthomasp amethodfordeterminingthedifferentialsedimentationofproteinsinthehighspeedconcentrationcentrifuge
AT pickelsedwardg amethodfordeterminingthedifferentialsedimentationofproteinsinthehighspeedconcentrationcentrifuge
AT horsfallfrankl amethodfordeterminingthedifferentialsedimentationofproteinsinthehighspeedconcentrationcentrifuge
AT hughesthomasp methodfordeterminingthedifferentialsedimentationofproteinsinthehighspeedconcentrationcentrifuge
AT pickelsedwardg methodfordeterminingthedifferentialsedimentationofproteinsinthehighspeedconcentrationcentrifuge
AT horsfallfrankl methodfordeterminingthedifferentialsedimentationofproteinsinthehighspeedconcentrationcentrifuge

Ejemplares similares