AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS

1. Antibody produced in the horse migrates as a new serum component between the β and γ components, whereas rabbit antibody is electrophoretically identical with the γ globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the γ globulin frac...

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Autores principales: Tiselius, Arne, Kabat, Elvin A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1939
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133726/
https://www.ncbi.nlm.nih.gov/pubmed/19870831
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author Tiselius, Arne
Kabat, Elvin A.
author_facet Tiselius, Arne
Kabat, Elvin A.
author_sort Tiselius, Arne
collection PubMed
description 1. Antibody produced in the horse migrates as a new serum component between the β and γ components, whereas rabbit antibody is electrophoretically identical with the γ globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the γ globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbed and absorbed sera. Antibody solutions of high purity can be obtained by electrophoretic isolation of the γ globulin of rabbit antisera in which the percentage of antibody to total γ globulin is high. 3. The isoelectric points of pig, cow, horse, and rabbit antibodies have been determined. 4. In horse sera prolonged immunization is accompanied by the formation of another antibody component of lower mobility.
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spelling pubmed-21337262008-04-18 AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS Tiselius, Arne Kabat, Elvin A. J Exp Med Article 1. Antibody produced in the horse migrates as a new serum component between the β and γ components, whereas rabbit antibody is electrophoretically identical with the γ globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the γ globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbed and absorbed sera. Antibody solutions of high purity can be obtained by electrophoretic isolation of the γ globulin of rabbit antisera in which the percentage of antibody to total γ globulin is high. 3. The isoelectric points of pig, cow, horse, and rabbit antibodies have been determined. 4. In horse sera prolonged immunization is accompanied by the formation of another antibody component of lower mobility. The Rockefeller University Press 1939-01-01 /pmc/articles/PMC2133726/ /pubmed/19870831 Text en Copyright © Copyright, 1939, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tiselius, Arne
Kabat, Elvin A.
AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS
title AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS
title_full AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS
title_fullStr AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS
title_full_unstemmed AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS
title_short AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS
title_sort electrophoretic study of immune sera and purified antibody preparations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133726/
https://www.ncbi.nlm.nih.gov/pubmed/19870831
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