ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA

A method of estimating the purity of preparations of elementary bodies of vaccinia is described. It depends on the comparison of the number of infective units of virus in a given material with the number of elementary bodies. The latter figure is estimated from the dry weight of the preparation by m...

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Detalles Bibliográficos
Autores principales: Smadel, Joseph E., Rivers, Thomas M., Pickels, Edward G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1939
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133794/
https://www.ncbi.nlm.nih.gov/pubmed/19870916
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author Smadel, Joseph E.
Rivers, Thomas M.
Pickels, Edward G.
author_facet Smadel, Joseph E.
Rivers, Thomas M.
Pickels, Edward G.
author_sort Smadel, Joseph E.
collection PubMed
description A method of estimating the purity of preparations of elementary bodies of vaccinia is described. It depends on the comparison of the number of infective units of virus in a given material with the number of elementary bodies. The latter figure is estimated from the dry weight of the preparation by means of a calculated value for the weight of a single dehydrated elementary body. Values for the ratio of infective units of vaccine virus to elementary bodies varied between 1 : 2.4 and 1 : 9.2 in seven consecutive experiments; the average was 1:4.2. These ratios indicate a high degree of purity of the preparation. Moreover, they indicate that a relatively high percentage of the elementary bodies in the preparations was infective.
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spelling pubmed-21337942008-04-18 ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA Smadel, Joseph E. Rivers, Thomas M. Pickels, Edward G. J Exp Med Article A method of estimating the purity of preparations of elementary bodies of vaccinia is described. It depends on the comparison of the number of infective units of virus in a given material with the number of elementary bodies. The latter figure is estimated from the dry weight of the preparation by means of a calculated value for the weight of a single dehydrated elementary body. Values for the ratio of infective units of vaccine virus to elementary bodies varied between 1 : 2.4 and 1 : 9.2 in seven consecutive experiments; the average was 1:4.2. These ratios indicate a high degree of purity of the preparation. Moreover, they indicate that a relatively high percentage of the elementary bodies in the preparations was infective. The Rockefeller University Press 1939-09-30 /pmc/articles/PMC2133794/ /pubmed/19870916 Text en Copyright © Copyright, 1939, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Smadel, Joseph E.
Rivers, Thomas M.
Pickels, Edward G.
ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA
title ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA
title_full ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA
title_fullStr ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA
title_full_unstemmed ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA
title_short ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA
title_sort estimation of the purity of preparations of elementary bodies of vaccinia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2133794/
https://www.ncbi.nlm.nih.gov/pubmed/19870916
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AT riversthomasm estimationofthepurityofpreparationsofelementarybodiesofvaccinia
AT pickelsedwardg estimationofthepurityofpreparationsofelementarybodiesofvaccinia

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