Microdomain Ca(2+) Activation during Exocytosis in Paramecium Cells. Superposition of Local Subplasmalemmal Calcium Store Activation by Local Ca(2+) Influx

In Paramecium tetraurelia, polyamine-triggered exocytosis is accompanied by the activation of Ca(2+)-activated currents across the cell membrane (Erxleben, C., and H. Plattner. 1994. J. Cell Biol. 127:935– 945). We now show by voltage clamp and extracellular recordings that the product of current × time (As) closely parallels the number of exocytotic events. We suggest that Ca(2+) mobilization from subplasmalemmal storage compartments, covering almost the entire cell surface, is a key event. In fact, after local stimulation, Ca(2+) imaging with high time resolution reveals rapid, transient, local signals even when extracellular Ca(2+) is quenched to or below resting intracellular Ca(2+) concentration ([Ca(2+)](e) ⩽ [Ca(2+)](i)). Under these conditions, quenched-flow/freeze-fracture analysis shows that membrane fusion is only partially inhibited. Increasing [Ca(2+)](e) alone, i.e., without secretagogue, causes rapid, strong cortical increase of [Ca(2+)](i) but no exocytosis. In various cells, the ratio of maximal vs. minimal currents registered during maximal stimulation or single exocytotic events, respectively, correlate nicely with the number of Ca stores available. Since no quantal current steps could be observed, this is again compatible with the combined occurrence of Ca(2+) mobilization from stores (providing close to threshold Ca(2+) levels) and Ca(2+) influx from the medium (which per se does not cause exocytosis). This implies that only the combination of Ca(2+) flushes, primarily from internal and secondarily from external sources, can produce a signal triggering rapid, local exocytotic responses, as requested for Paramecium defense.