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Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar
Between October 2001 and April 2002, five cases of acute flaccid paralysis (AFP) associated with type 2 vaccine-derived polioviruses (VDPVs) were reported in the southern province of the Republic of Madagascar. To determine viral factors that favor the emergence of these pathogenic VDPVs, we analyze...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2134956/ https://www.ncbi.nlm.nih.gov/pubmed/18085822 http://dx.doi.org/10.1371/journal.ppat.0030191 |
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author | Rakoto-Andrianarivelo, Mala Guillot, Sophie Iber, Jane Balanant, Jean Blondel, Bruno Riquet, Franck Martin, Javier Kew, Olen Randriamanalina, Bakolalao Razafinimpiasa, Lalatiana Rousset, Dominique Delpeyroux, Francis |
author_facet | Rakoto-Andrianarivelo, Mala Guillot, Sophie Iber, Jane Balanant, Jean Blondel, Bruno Riquet, Franck Martin, Javier Kew, Olen Randriamanalina, Bakolalao Razafinimpiasa, Lalatiana Rousset, Dominique Delpeyroux, Francis |
author_sort | Rakoto-Andrianarivelo, Mala |
collection | PubMed |
description | Between October 2001 and April 2002, five cases of acute flaccid paralysis (AFP) associated with type 2 vaccine-derived polioviruses (VDPVs) were reported in the southern province of the Republic of Madagascar. To determine viral factors that favor the emergence of these pathogenic VDPVs, we analyzed in detail their genomic and phenotypic characteristics and compared them with co-circulating enteroviruses. These VDPVs appeared to belong to two independent recombinant lineages with sequences from the type 2 strain of the oral poliovaccine (OPV) in the 5′-half of the genome and sequences derived from unidentified species C enteroviruses (HEV-C) in the 3′-half. VDPV strains showed characteristics similar to those of wild neurovirulent viruses including neurovirulence in poliovirus-receptor transgenic mice. We looked for other VDPVs and for circulating enteroviruses in 316 stools collected from healthy children living in the small area where most of the AFP cases occurred. We found vaccine PVs, two VDPVs similar to those found in AFP cases, some echoviruses, and above all, many serotypes of coxsackie A viruses belonging to HEV-C, with substantial genetic diversity. Several coxsackie viruses A17 and A13 carried nucleotide sequences closely related to the 2C and the 3D(pol) coding regions of the VDPVs, respectively. There was also evidence of multiple genetic recombination events among the HEV-C resulting in numerous recombinant genotypes. This indicates that co-circulation of HEV-C and OPV strains is associated with evolution by recombination, resulting in unexpectedly extensive viral diversity in small human populations in some tropical regions. This probably contributed to the emergence of recombinant VDPVs. These findings give further insight into viral ecosystems and the evolutionary processes that shape viral biodiversity. |
format | Text |
id | pubmed-2134956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-21349562007-12-27 Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar Rakoto-Andrianarivelo, Mala Guillot, Sophie Iber, Jane Balanant, Jean Blondel, Bruno Riquet, Franck Martin, Javier Kew, Olen Randriamanalina, Bakolalao Razafinimpiasa, Lalatiana Rousset, Dominique Delpeyroux, Francis PLoS Pathog Research Article Between October 2001 and April 2002, five cases of acute flaccid paralysis (AFP) associated with type 2 vaccine-derived polioviruses (VDPVs) were reported in the southern province of the Republic of Madagascar. To determine viral factors that favor the emergence of these pathogenic VDPVs, we analyzed in detail their genomic and phenotypic characteristics and compared them with co-circulating enteroviruses. These VDPVs appeared to belong to two independent recombinant lineages with sequences from the type 2 strain of the oral poliovaccine (OPV) in the 5′-half of the genome and sequences derived from unidentified species C enteroviruses (HEV-C) in the 3′-half. VDPV strains showed characteristics similar to those of wild neurovirulent viruses including neurovirulence in poliovirus-receptor transgenic mice. We looked for other VDPVs and for circulating enteroviruses in 316 stools collected from healthy children living in the small area where most of the AFP cases occurred. We found vaccine PVs, two VDPVs similar to those found in AFP cases, some echoviruses, and above all, many serotypes of coxsackie A viruses belonging to HEV-C, with substantial genetic diversity. Several coxsackie viruses A17 and A13 carried nucleotide sequences closely related to the 2C and the 3D(pol) coding regions of the VDPVs, respectively. There was also evidence of multiple genetic recombination events among the HEV-C resulting in numerous recombinant genotypes. This indicates that co-circulation of HEV-C and OPV strains is associated with evolution by recombination, resulting in unexpectedly extensive viral diversity in small human populations in some tropical regions. This probably contributed to the emergence of recombinant VDPVs. These findings give further insight into viral ecosystems and the evolutionary processes that shape viral biodiversity. Public Library of Science 2007-12 2007-12-14 /pmc/articles/PMC2134956/ /pubmed/18085822 http://dx.doi.org/10.1371/journal.ppat.0030191 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Rakoto-Andrianarivelo, Mala Guillot, Sophie Iber, Jane Balanant, Jean Blondel, Bruno Riquet, Franck Martin, Javier Kew, Olen Randriamanalina, Bakolalao Razafinimpiasa, Lalatiana Rousset, Dominique Delpeyroux, Francis Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar |
title | Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar |
title_full | Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar |
title_fullStr | Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar |
title_full_unstemmed | Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar |
title_short | Co-Circulation and Evolution of Polioviruses and Species C Enteroviruses in a District of Madagascar |
title_sort | co-circulation and evolution of polioviruses and species c enteroviruses in a district of madagascar |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2134956/ https://www.ncbi.nlm.nih.gov/pubmed/18085822 http://dx.doi.org/10.1371/journal.ppat.0030191 |
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