OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS

It has been shown experimentally that mixtures of two types of particles, namely, elementary bodies of vaccinia and collodion particles coated with protein, sediment with a single boundary in the analytical centrifuge. Such mixtures have been shown to develop one or two boundaries on electrophoresis...

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Autores principales: Smadel, J. E., Pickels, E. G., Shedlovsky, T., Rivers, T. M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1940
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135035/
https://www.ncbi.nlm.nih.gov/pubmed/19871041
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author Smadel, J. E.
Pickels, E. G.
Shedlovsky, T.
Rivers, T. M.
author_facet Smadel, J. E.
Pickels, E. G.
Shedlovsky, T.
Rivers, T. M.
author_sort Smadel, J. E.
collection PubMed
description It has been shown experimentally that mixtures of two types of particles, namely, elementary bodies of vaccinia and collodion particles coated with protein, sediment with a single boundary in the analytical centrifuge. Such mixtures have been shown to develop one or two boundaries on electrophoresis in the Tiselius apparatus, depending on the type of coating on the surface of the collodion particles. When covered with the heat-stable soluble antigen of vaccinia, collodion particles migrate in the electrical field at the same rate as elementary bodies. On the other hand, if they are coated with a component of normal rabbit serum, they migrate at a different rate. The estimation of purity of preparations of virus by means of data obtained by ultracentrifugation and electrophoresis is discussed.
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spelling pubmed-21350352008-04-18 OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS Smadel, J. E. Pickels, E. G. Shedlovsky, T. Rivers, T. M. J Exp Med Article It has been shown experimentally that mixtures of two types of particles, namely, elementary bodies of vaccinia and collodion particles coated with protein, sediment with a single boundary in the analytical centrifuge. Such mixtures have been shown to develop one or two boundaries on electrophoresis in the Tiselius apparatus, depending on the type of coating on the surface of the collodion particles. When covered with the heat-stable soluble antigen of vaccinia, collodion particles migrate in the electrical field at the same rate as elementary bodies. On the other hand, if they are coated with a component of normal rabbit serum, they migrate at a different rate. The estimation of purity of preparations of virus by means of data obtained by ultracentrifugation and electrophoresis is discussed. The Rockefeller University Press 1940-10-31 /pmc/articles/PMC2135035/ /pubmed/19871041 Text en Copyright © Copyright, 1940, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Smadel, J. E.
Pickels, E. G.
Shedlovsky, T.
Rivers, T. M.
OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS
title OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS
title_full OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS
title_fullStr OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS
title_full_unstemmed OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS
title_short OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS
title_sort observations on mixtures of elementary bodies of vaccinia and coated collodion particles by means of ultracentrifugation and electrophoresis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135035/
https://www.ncbi.nlm.nih.gov/pubmed/19871041
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AT shedlovskyt observationsonmixturesofelementarybodiesofvacciniaandcoatedcollodionparticlesbymeansofultracentrifugationandelectrophoresis
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