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THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES
An improved method is described for preparing the enzyme which hydrolyzes the polysaccharide acid contained in vitreous humor, umbilical cord, synovial fluid, and the mucoid phase of group A hemolytic streptococci. Preparations have been obtained from pneumococci, group A hemolytic streptococci, Clo...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1940
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135078/ https://www.ncbi.nlm.nih.gov/pubmed/19870951 |
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author | Meyer, Karl Hobby, Gladys L. Chaffee, Eleanor Dawson, Martin H. |
author_facet | Meyer, Karl Hobby, Gladys L. Chaffee, Eleanor Dawson, Martin H. |
author_sort | Meyer, Karl |
collection | PubMed |
description | An improved method is described for preparing the enzyme which hydrolyzes the polysaccharide acid contained in vitreous humor, umbilical cord, synovial fluid, and the mucoid phase of group A hemolytic streptococci. Preparations have been obtained from pneumococci, group A hemolytic streptococci, Clostridium welchii, and from splenic tissue, which display the same specific activity. Evidence is presented to show that the hydrolytic enzyme is not the same as that responsible for the lysis of pneumococci. In pneumococci and hemolytic streptococci the major portion of the enzyme is bound to the cell structure. The enzyme from Clostridium welchii is associated with other carbohydrate-splitting enzymes in the culture medium and not with the bacterial cells. It is suggested that the disappearance of the mucoid capsule of group A hemolytic streptococci is due to enzymatic hydrolysis of the acid polysaccharide. The relation between enzyme activity and the virulence and invasiveness of group A hemolytic streptococci is briefly considered. |
format | Text |
id | pubmed-2135078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1940 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21350782008-04-18 THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES Meyer, Karl Hobby, Gladys L. Chaffee, Eleanor Dawson, Martin H. J Exp Med Article An improved method is described for preparing the enzyme which hydrolyzes the polysaccharide acid contained in vitreous humor, umbilical cord, synovial fluid, and the mucoid phase of group A hemolytic streptococci. Preparations have been obtained from pneumococci, group A hemolytic streptococci, Clostridium welchii, and from splenic tissue, which display the same specific activity. Evidence is presented to show that the hydrolytic enzyme is not the same as that responsible for the lysis of pneumococci. In pneumococci and hemolytic streptococci the major portion of the enzyme is bound to the cell structure. The enzyme from Clostridium welchii is associated with other carbohydrate-splitting enzymes in the culture medium and not with the bacterial cells. It is suggested that the disappearance of the mucoid capsule of group A hemolytic streptococci is due to enzymatic hydrolysis of the acid polysaccharide. The relation between enzyme activity and the virulence and invasiveness of group A hemolytic streptococci is briefly considered. The Rockefeller University Press 1940-01-31 /pmc/articles/PMC2135078/ /pubmed/19870951 Text en Copyright © Copyright, 1940, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Meyer, Karl Hobby, Gladys L. Chaffee, Eleanor Dawson, Martin H. THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES |
title | THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES |
title_full | THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES |
title_fullStr | THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES |
title_full_unstemmed | THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES |
title_short | THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES |
title_sort | hydrolysis of hyaluronic acid by bacterial enzymes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135078/ https://www.ncbi.nlm.nih.gov/pubmed/19870951 |
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