THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE

A comparative study of spreading factor and hyaluronidase in preparations from various sources revealed the following points of similarity and dissimilarity in the two reactions. 1. Similarities: (a) All preparations containing hyaluronidase also produced spreading. (b) Heating at 65° and 100°C. for...

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Detalles Bibliográficos
Autores principales: Hobby, Gladys L., Dawson, Martin H., Meyer, Karl, Chaffee, Eleanor
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1941
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135114/
https://www.ncbi.nlm.nih.gov/pubmed/19871061
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author Hobby, Gladys L.
Dawson, Martin H.
Meyer, Karl
Chaffee, Eleanor
author_facet Hobby, Gladys L.
Dawson, Martin H.
Meyer, Karl
Chaffee, Eleanor
author_sort Hobby, Gladys L.
collection PubMed
description A comparative study of spreading factor and hyaluronidase in preparations from various sources revealed the following points of similarity and dissimilarity in the two reactions. 1. Similarities: (a) All preparations containing hyaluronidase also produced spreading. (b) Heating at 65° and 100°C. for 30 minutes produced a comparable effect on both reactions. (c) The demonstration of the presence of hyaluronic acid in skin offers a plausible explanation for the mechanism of spreading on the basis of hyaluronidase activity. 2. Dissimilarities: (a) No parallelism was observed in the degree of activity of spreading factor and hyaluronidase in the same preparations. (b) All preparations which produced spreading did not contain hyaluronidase. (c) Antisera to hyaluronidase preparations specifically and completely inhibited the activity of the homologous enzyme but did not inhibit the spreading factor in the same preparations. The significance of the similarities and dissimilarities between the two reactions is discussed. It is concluded that while hyaluronidase may play a rôle in the spreading reaction the phenomenon is a complex one and cannot be explained on the basis of a simple chemical reaction.
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spelling pubmed-21351142008-04-18 THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE Hobby, Gladys L. Dawson, Martin H. Meyer, Karl Chaffee, Eleanor J Exp Med Article A comparative study of spreading factor and hyaluronidase in preparations from various sources revealed the following points of similarity and dissimilarity in the two reactions. 1. Similarities: (a) All preparations containing hyaluronidase also produced spreading. (b) Heating at 65° and 100°C. for 30 minutes produced a comparable effect on both reactions. (c) The demonstration of the presence of hyaluronic acid in skin offers a plausible explanation for the mechanism of spreading on the basis of hyaluronidase activity. 2. Dissimilarities: (a) No parallelism was observed in the degree of activity of spreading factor and hyaluronidase in the same preparations. (b) All preparations which produced spreading did not contain hyaluronidase. (c) Antisera to hyaluronidase preparations specifically and completely inhibited the activity of the homologous enzyme but did not inhibit the spreading factor in the same preparations. The significance of the similarities and dissimilarities between the two reactions is discussed. It is concluded that while hyaluronidase may play a rôle in the spreading reaction the phenomenon is a complex one and cannot be explained on the basis of a simple chemical reaction. The Rockefeller University Press 1941-01-01 /pmc/articles/PMC2135114/ /pubmed/19871061 Text en Copyright © Copyright, 1941, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hobby, Gladys L.
Dawson, Martin H.
Meyer, Karl
Chaffee, Eleanor
THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE
title THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE
title_full THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE
title_fullStr THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE
title_full_unstemmed THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE
title_short THE RELATIONSHIP BETWEEN SPREADING FACTOR AND HYALURONIDASE
title_sort relationship between spreading factor and hyaluronidase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135114/
https://www.ncbi.nlm.nih.gov/pubmed/19871061
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