THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN

Methods are described for isolating a protein commonly present in the blood of patients during the acute phase of various infections which, unlike the normal serum proteins, is precipitable by the C polysaccharide of Pneumococcus. The reactive protein is present in the fraction of serum albumin prec...

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Detalles Bibliográficos
Autores principales: MacLeod, Colin M., Avery, Oswald T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1941
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135126/
https://www.ncbi.nlm.nih.gov/pubmed/19871071
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author MacLeod, Colin M.
Avery, Oswald T.
author_facet MacLeod, Colin M.
Avery, Oswald T.
author_sort MacLeod, Colin M.
collection PubMed
description Methods are described for isolating a protein commonly present in the blood of patients during the acute phase of various infections which, unlike the normal serum proteins, is precipitable by the C polysaccharide of Pneumococcus. The reactive protein is present in the fraction of serum albumin precipitated by either ammonium or sodium sulfate between 50 and 75 per cent saturation. From this fraction the reactive protein separates out on dialysis against tap water. Following removal of the alcohol-ether-soluble lipids from acute phase serum the reactive protein becomes soluble in tap water, and is no longer precipitable by traces of calcium but still retains its precipitability with the C polysaccharide.
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spelling pubmed-21351262008-04-18 THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN MacLeod, Colin M. Avery, Oswald T. J Exp Med Article Methods are described for isolating a protein commonly present in the blood of patients during the acute phase of various infections which, unlike the normal serum proteins, is precipitable by the C polysaccharide of Pneumococcus. The reactive protein is present in the fraction of serum albumin precipitated by either ammonium or sodium sulfate between 50 and 75 per cent saturation. From this fraction the reactive protein separates out on dialysis against tap water. Following removal of the alcohol-ether-soluble lipids from acute phase serum the reactive protein becomes soluble in tap water, and is no longer precipitable by traces of calcium but still retains its precipitability with the C polysaccharide. The Rockefeller University Press 1941-01-31 /pmc/articles/PMC2135126/ /pubmed/19871071 Text en Copyright © Copyright, 1941, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
MacLeod, Colin M.
Avery, Oswald T.
THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN
title THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN
title_full THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN
title_fullStr THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN
title_full_unstemmed THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN
title_short THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD : II. ISOLATION AND PROPERTIES OF THE REACTIVE PROTEIN
title_sort occurrence during acute infections of a protein not normally present in the blood : ii. isolation and properties of the reactive protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135126/
https://www.ncbi.nlm.nih.gov/pubmed/19871071
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