THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT

1. Methods for the separation from guinea pig serum in highly purified form of three of the components of complement are described. These substances are the so called mid-piece, end-piece, and 4th component. 2. Mid-piece has been separated as a euglobulin, with an electrophoretic mobility of 2.9 x 1...

Descripción completa

Detalles Bibliográficos
Autores principales: Pillemer, L., Ecker, E. E., Oncley, J. L., Cohn, E. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1941
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135187/
https://www.ncbi.nlm.nih.gov/pubmed/19871136
_version_ 1782142837921415168
author Pillemer, L.
Ecker, E. E.
Oncley, J. L.
Cohn, E. J.
author_facet Pillemer, L.
Ecker, E. E.
Oncley, J. L.
Cohn, E. J.
author_sort Pillemer, L.
collection PubMed
description 1. Methods for the separation from guinea pig serum in highly purified form of three of the components of complement are described. These substances are the so called mid-piece, end-piece, and 4th component. 2. Mid-piece has been separated as a euglobulin, with an electrophoretic mobility of 2.9 x 10(–5) in phosphate buffer of ionic strength 0.2 at pH 7.7, and with a sedimentation constant of 6.4 x 10(–13) in potassium chloride of ionic strength 0.2. 3. End-piece and 4th component were found together in a euglobulin fraction of serum which contained 10.3 per cent carbohydrate and had an electrophoretic mobility of 4.2 x 10(–5) in phosphate buffer of ionic strength 0.2 at pH 7.7.
format Text
id pubmed-2135187
institution National Center for Biotechnology Information
language English
publishDate 1941
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21351872008-04-18 THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT Pillemer, L. Ecker, E. E. Oncley, J. L. Cohn, E. J. J Exp Med Article 1. Methods for the separation from guinea pig serum in highly purified form of three of the components of complement are described. These substances are the so called mid-piece, end-piece, and 4th component. 2. Mid-piece has been separated as a euglobulin, with an electrophoretic mobility of 2.9 x 10(–5) in phosphate buffer of ionic strength 0.2 at pH 7.7, and with a sedimentation constant of 6.4 x 10(–13) in potassium chloride of ionic strength 0.2. 3. End-piece and 4th component were found together in a euglobulin fraction of serum which contained 10.3 per cent carbohydrate and had an electrophoretic mobility of 4.2 x 10(–5) in phosphate buffer of ionic strength 0.2 at pH 7.7. The Rockefeller University Press 1941-09-30 /pmc/articles/PMC2135187/ /pubmed/19871136 Text en Copyright © Copyright, 1941, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Pillemer, L.
Ecker, E. E.
Oncley, J. L.
Cohn, E. J.
THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT
title THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT
title_full THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT
title_fullStr THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT
title_full_unstemmed THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT
title_short THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT
title_sort preparation and physicochemical characterization of the serum protein components of complement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135187/
https://www.ncbi.nlm.nih.gov/pubmed/19871136
work_keys_str_mv AT pillemerl thepreparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement
AT eckeree thepreparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement
AT oncleyjl thepreparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement
AT cohnej thepreparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement
AT pillemerl preparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement
AT eckeree preparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement
AT oncleyjl preparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement
AT cohnej preparationandphysicochemicalcharacterizationoftheserumproteincomponentsofcomplement

Ejemplares similares