THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS

Studies on LS-protein, the soluble double antigen of vaccinia, and on the degradation products L'S and L''S' have been made with electrophoresis and in the analytical ultracentrifuge. LS, which is homogeneous electrically and in the ultracentrifuge, has an isoelectric point at pH...

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Detalles Bibliográficos
Autores principales: Shedlovsky, Theodore, Rothen, Alexandre, Smadel, Joseph E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1943
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135321/
https://www.ncbi.nlm.nih.gov/pubmed/19871271
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author Shedlovsky, Theodore
Rothen, Alexandre
Smadel, Joseph E.
author_facet Shedlovsky, Theodore
Rothen, Alexandre
Smadel, Joseph E.
author_sort Shedlovsky, Theodore
collection PubMed
description Studies on LS-protein, the soluble double antigen of vaccinia, and on the degradation products L'S and L''S' have been made with electrophoresis and in the analytical ultracentrifuge. LS, which is homogeneous electrically and in the ultracentrifuge, has an isoelectric point at pH 4.8. At 4°C. its partial specific volume is 0.72 cc./gm., and its diffusion constant is 1.50 x 10(–7) cm.(2)/sec. The sedimentation constant is 6.35 at 20°C., the molecular weight is 214,000, and the molecule appears to have an elongated ellipsoidal shape with an axis ratio of 1:20. L'S and L''S' are homogeneous electrically but not in the ultracentrifuge, L''S' being extremely polydisperse.
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spelling pubmed-21353212008-04-18 THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS Shedlovsky, Theodore Rothen, Alexandre Smadel, Joseph E. J Exp Med Article Studies on LS-protein, the soluble double antigen of vaccinia, and on the degradation products L'S and L''S' have been made with electrophoresis and in the analytical ultracentrifuge. LS, which is homogeneous electrically and in the ultracentrifuge, has an isoelectric point at pH 4.8. At 4°C. its partial specific volume is 0.72 cc./gm., and its diffusion constant is 1.50 x 10(–7) cm.(2)/sec. The sedimentation constant is 6.35 at 20°C., the molecular weight is 214,000, and the molecule appears to have an elongated ellipsoidal shape with an axis ratio of 1:20. L'S and L''S' are homogeneous electrically but not in the ultracentrifuge, L''S' being extremely polydisperse. The Rockefeller University Press 1943-02-01 /pmc/articles/PMC2135321/ /pubmed/19871271 Text en Copyright © Copyright, 1943, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Shedlovsky, Theodore
Rothen, Alexandre
Smadel, Joseph E.
THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS
title THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS
title_full THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS
title_fullStr THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS
title_full_unstemmed THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS
title_short THE LS-ANTIGEN OF VACCINIA : III. PHYSICAL-CHEMICAL PROPERTIES OF LS-ANTIGEN AND SOME OF ITS DEGRADATION PRODUCTS
title_sort ls-antigen of vaccinia : iii. physical-chemical properties of ls-antigen and some of its degradation products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135321/
https://www.ncbi.nlm.nih.gov/pubmed/19871271
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