STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN

1. Techniques for the preparation of skin antigen suitable for intramuscular injection in rabbits, and of skin antigens (autolysate) for serological experiments are described. 2. A method was evolved which produced a soluble skin antigen (autolysate) suitable for performing precipitin tests. 3. Inje...

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Detalles Bibliográficos
Autores principales: Hecht, Rudolph, Sulzberger, Marion B., Weil, Harry
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1943
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135363/
https://www.ncbi.nlm.nih.gov/pubmed/19871309
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author Hecht, Rudolph
Sulzberger, Marion B.
Weil, Harry
author_facet Hecht, Rudolph
Sulzberger, Marion B.
Weil, Harry
author_sort Hecht, Rudolph
collection PubMed
description 1. Techniques for the preparation of skin antigen suitable for intramuscular injection in rabbits, and of skin antigens (autolysate) for serological experiments are described. 2. A method was evolved which produced a soluble skin antigen (autolysate) suitable for performing precipitin tests. 3. Injection of the rabbit skin antigen and of staphylococcus toxin in rabbits resulted in the formation of antibodies (precipitins) to homologous skin. 4. When homologous skin alone was injected into rabbits, the antibody formation was questionable, or at most, slight. 5. The injection of staphylococcus toxin alone resulted in antibody formation, this antibody being specific for the toxin and not reacting with broth. 6. By utilization of the synergistic action of staphylococcus toxin and of homologous skin antigen, it has been possible for the first time to produce specific antiskin antibodies in experimental animals.
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spelling pubmed-21353632008-04-18 STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN Hecht, Rudolph Sulzberger, Marion B. Weil, Harry J Exp Med Article 1. Techniques for the preparation of skin antigen suitable for intramuscular injection in rabbits, and of skin antigens (autolysate) for serological experiments are described. 2. A method was evolved which produced a soluble skin antigen (autolysate) suitable for performing precipitin tests. 3. Injection of the rabbit skin antigen and of staphylococcus toxin in rabbits resulted in the formation of antibodies (precipitins) to homologous skin. 4. When homologous skin alone was injected into rabbits, the antibody formation was questionable, or at most, slight. 5. The injection of staphylococcus toxin alone resulted in antibody formation, this antibody being specific for the toxin and not reacting with broth. 6. By utilization of the synergistic action of staphylococcus toxin and of homologous skin antigen, it has been possible for the first time to produce specific antiskin antibodies in experimental animals. The Rockefeller University Press 1943-07-01 /pmc/articles/PMC2135363/ /pubmed/19871309 Text en Copyright © Copyright, 1943, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hecht, Rudolph
Sulzberger, Marion B.
Weil, Harry
STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN
title STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN
title_full STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN
title_fullStr STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN
title_full_unstemmed STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN
title_short STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF HOMOLOGOUS SKIN ANTIGEN AND STAPHYLOCOCCUS TOXIN
title_sort studies in sensitization to skin : i. the production of antibodies to skin by means of the synergistic action of homologous skin antigen and staphylococcus toxin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135363/
https://www.ncbi.nlm.nih.gov/pubmed/19871309
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