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PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI
1. Strains of staphylococci produce diazotizable materials which can be converted to a dye, and the intensity of the color reaction can be quantitated in the same manner as p-aminobenzoic acid. The sulfonamide-resistant strains which we have studied produce more diazotizable substance than non-resis...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1944
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135388/ https://www.ncbi.nlm.nih.gov/pubmed/19871374 |
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author | Spink, Wesley W. Wright, Lemuel D. Vivino, Jean J. Skeggs, Helen R. |
author_facet | Spink, Wesley W. Wright, Lemuel D. Vivino, Jean J. Skeggs, Helen R. |
author_sort | Spink, Wesley W. |
collection | PubMed |
description | 1. Strains of staphylococci produce diazotizable materials which can be converted to a dye, and the intensity of the color reaction can be quantitated in the same manner as p-aminobenzoic acid. The sulfonamide-resistant strains which we have studied produce more diazotizable substance than non-resistant strains. 2. The development of a color by the diazotizable substance can be inhibited by exposing the substance to a soil bacillus (Mirick) specifically adapted to oxidize PABA. 3. The diazotizable substance produced by staphylococci inhibits the anti-staphylococcic action of sodium sulfathiazole to approximately the same degree as equivalent amounts of pure p-aminobenzoic acid. 4. Two microbiological methods for assaying p-aminobenzoic acid were employed for quantitating the amount of this material produced by staphylococci. In general, the sulfonamide-resistant strains produced more p-aminobenzoic acid than the sulfonamide-sensitive strains. The inconstant results obtained with these biological assays are discussed. |
format | Text |
id | pubmed-2135388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1944 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21353882008-04-18 PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI Spink, Wesley W. Wright, Lemuel D. Vivino, Jean J. Skeggs, Helen R. J Exp Med Article 1. Strains of staphylococci produce diazotizable materials which can be converted to a dye, and the intensity of the color reaction can be quantitated in the same manner as p-aminobenzoic acid. The sulfonamide-resistant strains which we have studied produce more diazotizable substance than non-resistant strains. 2. The development of a color by the diazotizable substance can be inhibited by exposing the substance to a soil bacillus (Mirick) specifically adapted to oxidize PABA. 3. The diazotizable substance produced by staphylococci inhibits the anti-staphylococcic action of sodium sulfathiazole to approximately the same degree as equivalent amounts of pure p-aminobenzoic acid. 4. Two microbiological methods for assaying p-aminobenzoic acid were employed for quantitating the amount of this material produced by staphylococci. In general, the sulfonamide-resistant strains produced more p-aminobenzoic acid than the sulfonamide-sensitive strains. The inconstant results obtained with these biological assays are discussed. The Rockefeller University Press 1944-04-01 /pmc/articles/PMC2135388/ /pubmed/19871374 Text en Copyright © Copyright, 1944, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Spink, Wesley W. Wright, Lemuel D. Vivino, Jean J. Skeggs, Helen R. PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI |
title | PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI |
title_full | PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI |
title_fullStr | PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI |
title_full_unstemmed | PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI |
title_short | PARA-AMINOBENZOIC ACID PRODUCTION BY STAPHYLOCOCCI |
title_sort | para-aminobenzoic acid production by staphylococci |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135388/ https://www.ncbi.nlm.nih.gov/pubmed/19871374 |
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