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AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS

1. The present paper constitutes a preliminary study of the morphology of mitochondria by means of electron microscopy. 2. The mitochondria that were the subject of this investigation were obtained from a lymphosarcoma of the rat. They were separated from the other components of the leukemic cells b...

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Detalles Bibliográficos
Autores principales: Claude, Albert, Fullam, Ernest F.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1945
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135528/
https://www.ncbi.nlm.nih.gov/pubmed/19871443
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author Claude, Albert
Fullam, Ernest F.
author_facet Claude, Albert
Fullam, Ernest F.
author_sort Claude, Albert
collection PubMed
description 1. The present paper constitutes a preliminary study of the morphology of mitochondria by means of electron microscopy. 2. The mitochondria that were the subject of this investigation were obtained from a lymphosarcoma of the rat. They were separated from the other components of the leukemic cells by a method of differential centrifugation, and thus made available for direct examination in the electron microscope. 3. In the purified form the mitochondria appeared as spherical bodies, the majority of them varying in size approximately from 0.6 to 1.3 µ in diameter. 4. Certain aspects of mitochondria in the electron microscope suggest that these elements are surrounded by a differentiated membrane. In some cases the limiting membrane seemed to be responsible for maintaining the general shape of the mitochondria, even when most of the mitochondrial substance had been lost. 5. By means of the electron microscope, it is possible to distinguish small elements, 80 to 100 mµ in diameter, within the body of certain mitochondria. Further work is suggested to establish whether these small granules are normal constituents of mitochondria, and what relation may exist between them and ordinary microsomes. 6. The nature of mitochondria as morphological units is discussed. Present evidence indicates that mitochondria constitute definite physical entities which can persist in the absence of the cytoplasm.
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spelling pubmed-21355282008-04-18 AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS Claude, Albert Fullam, Ernest F. J Exp Med Article 1. The present paper constitutes a preliminary study of the morphology of mitochondria by means of electron microscopy. 2. The mitochondria that were the subject of this investigation were obtained from a lymphosarcoma of the rat. They were separated from the other components of the leukemic cells by a method of differential centrifugation, and thus made available for direct examination in the electron microscope. 3. In the purified form the mitochondria appeared as spherical bodies, the majority of them varying in size approximately from 0.6 to 1.3 µ in diameter. 4. Certain aspects of mitochondria in the electron microscope suggest that these elements are surrounded by a differentiated membrane. In some cases the limiting membrane seemed to be responsible for maintaining the general shape of the mitochondria, even when most of the mitochondrial substance had been lost. 5. By means of the electron microscope, it is possible to distinguish small elements, 80 to 100 mµ in diameter, within the body of certain mitochondria. Further work is suggested to establish whether these small granules are normal constituents of mitochondria, and what relation may exist between them and ordinary microsomes. 6. The nature of mitochondria as morphological units is discussed. Present evidence indicates that mitochondria constitute definite physical entities which can persist in the absence of the cytoplasm. The Rockefeller University Press 1945-01-01 /pmc/articles/PMC2135528/ /pubmed/19871443 Text en Copyright © Copyright, 1945, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Claude, Albert
Fullam, Ernest F.
AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS
title AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS
title_full AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS
title_fullStr AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS
title_full_unstemmed AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS
title_short AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS
title_sort electron microscope study of isolated mitochondria : method and preliminary results
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135528/
https://www.ncbi.nlm.nih.gov/pubmed/19871443
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