IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY

1. The microquantitative precipitin method can be used to compare the relative activity of different preparations of the blood group A substance from hog stomachs and to study the effect of chemical treatment upon its stability. 2. With samples of about 25 µg. antibody nitrogen, an error of ±1.7 µg....

Descripción completa

Detalles Bibliográficos
Autores principales: Kabat, Elvin A., Bendich, Aaron, Bezer, Ada E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1946
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135588/
https://www.ncbi.nlm.nih.gov/pubmed/19871544
_version_ 1782142930990923776
author Kabat, Elvin A.
Bendich, Aaron
Bezer, Ada E.
author_facet Kabat, Elvin A.
Bendich, Aaron
Bezer, Ada E.
author_sort Kabat, Elvin A.
collection PubMed
description 1. The microquantitative precipitin method can be used to compare the relative activity of different preparations of the blood group A substance from hog stomachs and to study the effect of chemical treatment upon its stability. 2. With samples of about 25 µg. antibody nitrogen, an error of ±1.7 µg. antibody nitrogen will result in an error of ±12 per cent in the estimation of the amount of A substance. 3. The blood group A substance showed no significant loss of activity at 37°C. after 48 hours at pH 1.07 to 10.7 or after 2 hours at 100°C. over a pH range from 2.97 to 7.58. Exposure at 100°C. at pH 1.03 or at 9.03 or higher resulted in loss of activity. Parallel results were obtained by the hemagglutination inhibition and quantitative precipitin methods. 4. The solubility of specific precipitates of the blood group A substance from hog stomach and its homologous antibody formed in man was found to be about 1.6 µg. antibody N/ml. 5. A comparison is given of the chemical properties and activity of blood group A substances obtained by several procedures from pools of hog stomachs.
format Text
id pubmed-2135588
institution National Center for Biotechnology Information
language English
publishDate 1946
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21355882008-04-18 IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY Kabat, Elvin A. Bendich, Aaron Bezer, Ada E. J Exp Med Article 1. The microquantitative precipitin method can be used to compare the relative activity of different preparations of the blood group A substance from hog stomachs and to study the effect of chemical treatment upon its stability. 2. With samples of about 25 µg. antibody nitrogen, an error of ±1.7 µg. antibody nitrogen will result in an error of ±12 per cent in the estimation of the amount of A substance. 3. The blood group A substance showed no significant loss of activity at 37°C. after 48 hours at pH 1.07 to 10.7 or after 2 hours at 100°C. over a pH range from 2.97 to 7.58. Exposure at 100°C. at pH 1.03 or at 9.03 or higher resulted in loss of activity. Parallel results were obtained by the hemagglutination inhibition and quantitative precipitin methods. 4. The solubility of specific precipitates of the blood group A substance from hog stomach and its homologous antibody formed in man was found to be about 1.6 µg. antibody N/ml. 5. A comparison is given of the chemical properties and activity of blood group A substances obtained by several procedures from pools of hog stomachs. The Rockefeller University Press 1946-05-31 /pmc/articles/PMC2135588/ /pubmed/19871544 Text en Copyright © Copyright, 1946, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kabat, Elvin A.
Bendich, Aaron
Bezer, Ada E.
IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_full IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_fullStr IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_full_unstemmed IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_short IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_sort immunochemical studies on blood groups : ii. properties of the blood group a substance from pools of hoo stomachs and of specific precipitates composed of "a" substance and homologous human antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135588/
https://www.ncbi.nlm.nih.gov/pubmed/19871544
work_keys_str_mv AT kabatelvina immunochemicalstudiesonbloodgroupsiipropertiesofthebloodgroupasubstancefrompoolsofhoostomachsandofspecificprecipitatescomposedofasubstanceandhomologoushumanantibody
AT bendichaaron immunochemicalstudiesonbloodgroupsiipropertiesofthebloodgroupasubstancefrompoolsofhoostomachsandofspecificprecipitatescomposedofasubstanceandhomologoushumanantibody
AT bezeradae immunochemicalstudiesonbloodgroupsiipropertiesofthebloodgroupasubstancefrompoolsofhoostomachsandofspecificprecipitatescomposedofasubstanceandhomologoushumanantibody

Ejemplares similares