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THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES

The effect of ultraviolet irradiation on various properties of the influenza viruses Types A and B has been analyzed. The studies involved propagation and interference in the allantoic sac of the chick embryo, inhibition of embryonic development, toxicity for white mice, hemagglutination including t...

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Detalles Bibliográficos
Autores principales: Henle, Werner, Henle, Gertrude
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1947
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135615/
https://www.ncbi.nlm.nih.gov/pubmed/19871620
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author Henle, Werner
Henle, Gertrude
author_facet Henle, Werner
Henle, Gertrude
author_sort Henle, Werner
collection PubMed
description The effect of ultraviolet irradiation on various properties of the influenza viruses Types A and B has been analyzed. The studies involved propagation and interference in the allantoic sac of the chick embryo, inhibition of embryonic development, toxicity for white mice, hemagglutination including the adsorption-elution mechanism, immunizing capacity for mice and, finally, complement fixation activities in the presence of antibodies to the 600S antigen (human convalescent and postvaccination sera) and the 30S antigen (convalescent sera only). It has been shown that the various activities of the influenza viruses were affected by irradiation at different rates, indicating that they are based, at least in part, on different constituents of the virus particle. On account of these differences in the susceptibility of the various properties to ultraviolet light it was possible (a) to differentiate between the interference phenomenon as observed in the allantoic sac, and the development of non-agglutinability in red cells by either homologous or heterologous fresh virus, and (b) to separate individual steps involved in the mechanism of infection of susceptible host cells. The implications of these findings are discussed.
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spelling pubmed-21356152008-04-18 THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES Henle, Werner Henle, Gertrude J Exp Med Article The effect of ultraviolet irradiation on various properties of the influenza viruses Types A and B has been analyzed. The studies involved propagation and interference in the allantoic sac of the chick embryo, inhibition of embryonic development, toxicity for white mice, hemagglutination including the adsorption-elution mechanism, immunizing capacity for mice and, finally, complement fixation activities in the presence of antibodies to the 600S antigen (human convalescent and postvaccination sera) and the 30S antigen (convalescent sera only). It has been shown that the various activities of the influenza viruses were affected by irradiation at different rates, indicating that they are based, at least in part, on different constituents of the virus particle. On account of these differences in the susceptibility of the various properties to ultraviolet light it was possible (a) to differentiate between the interference phenomenon as observed in the allantoic sac, and the development of non-agglutinability in red cells by either homologous or heterologous fresh virus, and (b) to separate individual steps involved in the mechanism of infection of susceptible host cells. The implications of these findings are discussed. The Rockefeller University Press 1947-03-31 /pmc/articles/PMC2135615/ /pubmed/19871620 Text en Copyright © Copyright, 1947, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Henle, Werner
Henle, Gertrude
THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES
title THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES
title_full THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES
title_fullStr THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES
title_full_unstemmed THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES
title_short THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES
title_sort effect of ultraviolet irradiation on various properties of influenza viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135615/
https://www.ncbi.nlm.nih.gov/pubmed/19871620
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